Vogl A W
Department of Anatomy, Faculty of Medicine, University of British Columbia, Vancouver, Canada.
Anat Rec. 1988 Sep;222(1):34-41. doi: 10.1002/ar.1092220107.
I have studied the distribution of microtubules in rat Sertoli cells during spermatogenesis. The distribution of microtubules was determined by indirect immunofluorescence in fragments of seminiferous epithelium which were mechanically dissociated from perfusion-fixed testes. The presence of microtubules in regions indicated by immunofluorescence was confirmed with electron microscopy. Microtubules are not evident in Sertoli cell regions surrounding early spermatogenic cells, but are abundant in cytoplasm adjacent to apical crypts containing elongate spermatids. In regions surrounding crypts, microtubules are uniformly aligned parallel to the long axis of Sertoli cells. Microtubules in these more central, or columnar, regions of the cells extend from supranuclear levels to the apex of the epithelium. In apical processes surrounding late spermatids, microtubules conform to the contours of the hook-shaped spermatid heads. These microtubules appear continuous with those in the columnar regions of the Sertoli cells. Changes in microtubule distribution observed in the rat are generally similar to, but less elaborate than, those reported previously in the squirrel. These changes may be characteristic of mammalian spermatogenesis in general.
我研究了大鼠支持细胞在精子发生过程中微管的分布情况。微管的分布是通过间接免疫荧光法在从灌注固定的睾丸中机械分离出的生精上皮片段中确定的。免疫荧光显示区域中微管的存在通过电子显微镜得到了证实。在围绕早期生精细胞的支持细胞区域中微管不明显，但在与含有伸长型精子细胞的顶端隐窝相邻的细胞质中微管丰富。在隐窝周围区域，微管均匀地平行于支持细胞的长轴排列。在细胞这些更靠中心或柱状的区域中，微管从核上水平延伸至上皮顶端。在围绕晚期精子细胞的顶端突起中，微管与钩状精子细胞头部的轮廓相符。这些微管似乎与支持细胞柱状区域中的微管相连。在大鼠中观察到的微管分布变化通常与之前在松鼠中报道的相似，但不如其精细。这些变化可能是一般哺乳动物精子发生的特征。
