Istituto Zooprofilattico Sperimentale Umbria e Marche "Togo Rosati", via G. Salvemini 1, 06126 Perugia, Italy.
National Reference Laboratory for Coagulase Positive Staphylococci - Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, via Bologna 148, 10154 Torino, Italy.
Toxins (Basel). 2018 Dec 6;10(12):523. doi: 10.3390/toxins10120523.
In summer 2017, a foodborne outbreak occurred in Central Italy, involving 26 workers employed in the post-earthquake reconstruction. After eating a meal provided by a catering service, they manifested gastrointestinal symptoms; 23 of them were hospitalized. The retrospective cohort study indicated the pasta salad as the most likely vehicle of poisoning. Foods, environmental samples, and food handlers' nasal swabs were collected. () and coagulase-positive staphylococci (CPS) including , together with their toxins, were the targets of the analysis. CPS, detected in all the leftovers, exceeded 10⁵ CFU/g in the pasta salad, in which we found Staphylococcal Enterotoxins (SEs) (0.033 ng SEA/g; 0.052 ng SED/g). None of the environmental and human swabs showed contamination. We characterized 23 from foods. They all belonged to the human biotype, showed the same toxigenic profile (, , , and genes), and had the same Pulsed Field Gel Electrophoresis (PFGE) pattern; none of them harbored or genes. We also detected contamination in the pasta salad but none of the isolates harbored the gene for the emetic toxin cereulide. The EU Reference Laboratory for CPS confirmed the case as a strong-evidence outbreak caused by the ingestion of SEs produced by a single strain of carried by the same human source. This outbreak was successfully investigated despite the emergency situation in which it occurred.
2017 年夏天，意大利中部发生了一起食源性疾病暴发事件，涉及 26 名参与地震灾后重建的工人。他们在食用了由餐饮服务提供的餐后出现了胃肠道症状，其中 23 人住院。回顾性队列研究表明，通心粉沙拉是最有可能导致中毒的食物。采集了食品、环境样本和食品处理人员的鼻拭子。分析的目标是金黄色葡萄球菌（ ）和凝固酶阳性葡萄球菌（CPS），包括 ，以及它们的毒素。在所有剩余食物中均检测到 CPS，通心粉沙拉中的 CPS 超过 10⁵CFU/g，我们在其中发现了葡萄球菌肠毒素（SEs）（0.033ngSEA/g；0.052ngSED/g）。环境和人体拭子均未显示污染。我们从食物中鉴定出 23 株 。它们均属于人源生物型，具有相同的产毒谱（ 、 、 、和 基因），脉冲场凝胶电泳（PFGE）图谱相同；它们均不携带 或 基因。我们还在通心粉沙拉中检测到了 污染，但没有分离株携带呕吐毒素cereulide 的基因。欧盟 CPS 参考实验室确认该病例为一起强烈证据暴发事件，由同一来源携带的单一 菌株产生的 SEs 摄入引起。尽管暴发发生在紧急情况下，但仍成功进行了调查。
