Roth G J, Ozols J, Nugent D J, Williams S A
Medical Service, Veterans Administration Medical Center, Seattle, WA 98108.
Biochem Biophys Res Commun. 1988 Oct 31;156(2):931-9. doi: 10.1016/s0006-291x(88)80933-1.
Human platelet glycoprotein IX is a small (Mr 17,000) surface glycoprotein present in the plasma membrane as a 1:1 non-covalent complex with glycoprotein Ib (Mr 165,000). Glycoprotein IX was purified to near homogeneity by sequential wheat germ agglutinin and immuno-affinity chromatography, followed by gel filtration chromatography under denaturing conditions. Purified glycoprotein IX was characterized by determination of its amino acid composition and its NH2-terminal amino acid sequence (thr-lys-asp-xxx-pro-ser-xxx-leu-thr-(thr)2-arg-ala-(leu)-(glu)-xxx-met- gly), and monospecific anti-glycoprotein IX antibody was prepared. The study outlines a useful approach for separating and characterizing glycoprotein IX, free from other members of the glycoprotein Ib complex.
人血小板糖蛋白IX是一种小分子（分子量17,000）表面糖蛋白，以与糖蛋白Ib（分子量165,000）形成1:1非共价复合物的形式存在于质膜中。通过依次进行麦胚凝集素亲和层析和免疫亲和层析，然后在变性条件下进行凝胶过滤层析，将糖蛋白IX纯化至近乎同质。通过测定其氨基酸组成及其NH2末端氨基酸序列（苏氨酸-赖氨酸-天冬氨酸-xxx-脯氨酸-丝氨酸-xxx-亮氨酸-苏氨酸-（苏氨酸）2-精氨酸-丙氨酸-（亮氨酸）-（谷氨酸）-xxx-甲硫氨酸-甘氨酸）对纯化的糖蛋白IX进行了表征，并制备了单特异性抗糖蛋白IX抗体。该研究概述了一种分离和表征糖蛋白IX的有用方法，该方法可使其与糖蛋白Ib复合物的其他成员分离。
