Werner Christina, Sauerwald Claudia, Sundrum Albert, El-Sayed Amr, Zschöck Michael
Department of Animal Nutrition and Animal Health, Faculty of Organic Agricultural Sciences, University of Kassel, D-37213 Witzenhausen, Germany.
Food and Veterinary Office, District of Waldeck - Frankenberg, D-35066 Frankenberg, Germany.
Int J Vet Sci Med. 2018 Oct 16;6(2):274-278. doi: 10.1016/j.ijvsm.2018.09.005. eCollection 2018 Dec.
In the present work, macrorestriction analysis was applied to characterize 44 field strains isolated from lactating cows suffering from mastitis in three dairy herds in Hesse State, Germany. Analysis of the obtained data by Pulse-Field Gel Electrophoresis (PFGE) showed that most of the isolates originating from different herds and cows were not related to each other. However, identical macrorestriction patterns were noted in 12 of 13 mastitic quarters in healing process, in three quarters even over the whole sampling period indicating persistent infection. In the present work, PFGE could detect variable levels of similarity ranging from 76 to 100%. The macrorestriction analyses revealed the presence of 10 PFGE pattern with more than four bands difference. PFGE profiles with minor differences (only one to three bands) were considered to be subtypes. The use of sensitive genotyping methods like macrorestriction analyses by PFGE enables the differentiation among new and persistent infections. Nevertheless minor changes in macrorestriction profiles could occur which are clearly distinguishable from totally unrelated strains.
在本研究中，采用宏观限制性分析对从德国黑森州三个奶牛场患乳腺炎的泌乳奶牛中分离出的44株现场菌株进行了特征分析。通过脉冲场凝胶电泳（PFGE）对所得数据进行分析表明，大多数来自不同牛群和奶牛的分离株彼此不相关。然而，在13个处于愈合过程的患乳腺炎乳腺区中的12个中，甚至在整个采样期间的三个乳腺区中，观察到相同的宏观限制性图谱，表明存在持续性感染。在本研究中，PFGE能够检测到76%至100%的不同相似度水平。宏观限制性分析揭示了存在10种具有超过四条带差异的PFGE图谱。具有微小差异（仅一至三条带）的PFGE图谱被认为是亚型。使用像PFGE宏观限制性分析这样的灵敏基因分型方法能够区分新感染和持续性感染。然而，宏观限制性图谱可能会发生微小变化，这与完全不相关的菌株明显不同。
