果蝇成虫盘中介导Hippo信号通路的组件的实时成像

Live Imaging of Hippo Pathway Components in Drosophila Imaginal Discs.

作者信息

Xu Jiajie, Su Ting, Tokamov Sherzod A, Fehon Richard G

机构信息

Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, IL, USA.

Committee on Development, Regeneration and Stem Cell Biology, The University of Chicago, Chicago, IL, USA.

出版信息

Methods Mol Biol. 2019;1893:53-59. doi: 10.1007/978-1-4939-8910-2_4.

DOI:10.1007/978-1-4939-8910-2_4

PMID:30565124

Abstract

Examining the subcellular localization of Hippo pathway components has helped elucidate the molecular mechanisms that regulate the pathway. Here we describe methods for performing live imaging of fluorescently tagged Hippo pathway components in Drosophila wing imaginal discs.

摘要

研究Hippo信号通路组分的亚细胞定位有助于阐明调控该信号通路的分子机制。在此，我们描述了在果蝇翅成虫盘（wing imaginal discs）中对荧光标记的Hippo信号通路组分进行实时成像的方法。