State Key Laboratory of Genetic Engineering and Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, School of Life Sciences, Fudan University, Shanghai, 200438, China.
Laboratory of Population & Quantitative Genetics, Institute of Genetics and Biostatistics, School of Life Sciences, Fudan University, Shanghai, 200438, China.
J Integr Plant Biol. 2019 Nov;61(11):1121-1127. doi: 10.1111/jipb.12761. Epub 2019 Feb 27.
Mimicry target-directed microRNA degradation is widespread and highly conserved among eukaryotes. However, little is known about its mechanism of action. In this letter, by using STTM160 (target mimic of miR160) as a reporter, we show that dysfunction of HAWAIIAN SKIRT (HWS) suppresses the pleiotropic phenotype of STTM160. Small RNA sequencing and Northern blot analyses suggested that HWS only affects a subset of microRNAs. Intriguingly, we identified a stable coexistence of miR160/miR399 and their mimicry targets within the AGO1 complex when HWS is compromised, pointing to a possible role of HWS in the clearance of RNA-induced silencing complexes associated with mimicry target.
拟态靶向 microRNA 降解在真核生物中广泛存在且高度保守。然而，其作用机制尚不清楚。在这封信中，我们使用 STTM160（miR160 的靶标模拟物）作为报告物，表明 HAWAIIAN SKIRT（HWS）功能障碍抑制了 STTM160 的多效表型。小 RNA 测序和 Northern blot 分析表明，HWS 仅影响一小部分 microRNAs。有趣的是，当 HWS 受损时，我们在 AGO1 复合物中鉴定到 miR160/miR399 及其模拟物靶标的稳定共存，这表明 HWS 可能在清除与模拟物靶标相关的 RNA 诱导沉默复合物中发挥作用。
