Yasuhara-Bell Jarred, Pieck Michael L, Ruck Amy, Farman Mark L, Peterson Gary L, Stack James P, Valent Barbara, Pedley Kerry F
1 Department of Plant Pathology, Kansas State University, Throckmorton Plant Sciences Center, Manhattan, KS 66506.
2 United States Department of Agriculture-Agricultural Research Service (USDA-ARS), Foreign Disease-Weed Science Research Unit (FDWSRU), Ft. Detrick, MD 21702; and.
Phytopathology. 2019 Apr;109(4):509-511. doi: 10.1094/PHYTO-10-18-0397-LE. Epub 2019 Mar 20.
This is a response to a recent Letter to the Editor of Phytopathology, in which Gupta et al. (2019) caution against the indiscriminate use of the MoT3 diagnostic assay that distinguishes isolates of Magnaporthe oryzae in the Triticum lineage from those that do not cause aggressive wheat blast. We confirm that the assay does reliably distinguish between wheat and rice isolates from Bangladesh and worldwide, as described in the original paper by Pieck et al. (2017) . We have been unable to reproduce the equally intense amplification of WB12 and WB12-like sequences reported in Figure 1 of the Letter. Other data presented by Gupta et al. (2019) support the specificity of the MoT3 assay. Therefore, cautions beyond those always associated with accurate reproduction of diagnostic assays are unwarranted.
这是对最近发表在《植物病理学》杂志上给编辑的一封信的回应，Gupta等人（2019年）在信中告诫人们不要不加区分地使用MoT3诊断检测方法，该方法用于区分小麦谱系中的稻瘟病菌分离株与那些不会引发侵袭性小麦叶瘟的分离株。正如Pieck等人（2017年）在原论文中所描述的，我们证实该检测方法确实能够可靠地区分来自孟加拉国及世界各地的小麦和水稻分离株。我们无法重现该信图1中所报道的WB12和WB12样序列同等强度的扩增。Gupta等人（2019年）提供的其他数据支持了MoT3检测方法的特异性。因此，除了那些与诊断检测的准确重现始终相关的注意事项之外，无需再作其他告诫。
