MOE Key Laboratory of Bioinorganic and Synthetic Chemistry/KLGHEI of Environment and Energy Chemistry, School of Chemistry, Sun Yat-Sen University, Guangzhou, 510275, China.
MOE Key Laboratory of Bioinorganic and Synthetic Chemistry/KLGHEI of Environment and Energy Chemistry, School of Chemistry, Sun Yat-Sen University, Guangzhou, 510275, China.
Anal Chim Acta. 2019 Jan 24;1047:62-70. doi: 10.1016/j.aca.2018.09.060. Epub 2018 Sep 28.
Antibiotics are a group of antibacterial drugs used in the treatment and prevention of bacterial diseases in humans, veterinary animals, and farmed fish. Inappropriate and excessive use of antibiotic therapy leads to antibiotic fragment in the water bodies thus affect the aquatic organisms. In this study, an amino group modified high surface area metal-organic framework (MOF) MIL-101(Cr)-NH was used to prepare solid-phase microextraction (SPME) fiber to detect four different classes (total 6 ABs) of antibiotics for the first time from living tilapia fish (Oreochromis mossambicus) by coupling SPME with high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS). The extraction efficiencies of the custom-made fiber were superior as compared with the commercial C18, polydimethylsiloxane (PDMS), PDMS/divinylbenzene (DVB) and polyacrylate fibers. The custom-made fiber also exhibited excellent reproducibility with the low intra-fiber relative standard deviations (RSDs 1.5%-8.3%) and inter-fiber RSDs (7.3%-14.5%), which made it ideal for in vivo extraction in fish muscle. The as-prepared MIL-101(Cr)-NH fiber was then used to determine antibiotics in the dorsal-epaxial muscle of living fish. Comparing to the traditional solid-liquid extraction (SLE) method, the SPME method showed reduced invasiveness and higher sensitivity than the SLE method. In general, this study explored a convenient, cost-effective and highly sensitive SPME method based on amino modified MOF for in vivo antibiotic detection in fish muscle.
抗生素是一组用于治疗和预防人类、兽医动物和养殖鱼类细菌疾病的抗菌药物。抗生素治疗的不适当和过度使用会导致水体中的抗生素残留，从而影响水生生物。在这项研究中，首次使用氨基功能化的高比表面积金属有机骨架（MOF） MIL-101（Cr）-NH 制备固相微萃取（SPME）纤维，通过与高效液相色谱-串联质谱（LC-MS/MS）联用，从活体罗非鱼（Oreochromis mossambicus）中检测四种不同类别的抗生素（共 6 种 ABs）。与商用 C18、聚二甲基硅氧烷（PDMS）、PDMS/二乙烯基苯（DVB）和聚丙烯酸酯纤维相比，定制纤维的萃取效率更高。自制纤维还表现出出色的重现性，其纤维内相对标准偏差（RSDs 为 1.5%-8.3%）和纤维间 RSDs（7.3%-14.5%）均较低，非常适合在鱼肌肉内进行活体萃取。然后，将制备的 MIL-101（Cr）-NH 纤维用于检测活体鱼背鳍-背侧肌中的抗生素。与传统的固液萃取（SLE）方法相比，SPME 方法具有较低的侵入性和更高的灵敏度。总的来说，本研究探索了一种基于氨基改性 MOF 的方便、经济高效且高灵敏度的 SPME 方法，用于在鱼肌肉中进行活体抗生素检测。
