Khoshnevis Nika, Rezaei Shahla, Samaei-Nouroozi Amene, Amin Mohsen, Moshfegh Mahsa, Khoshayand Mohammad Reza, Faramarzi Mohammad Ali
Department of Pharmaceutical Biotechnology, Faculty of Pharmacy and Biotechnology Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Department of Drug and Food Control, Faculty of Pharmacy and Pharmaceuticals Quality Assurance Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Iran J Pharm Res. 2018 Fall;17(4):1392-1412.
Owing to their superior catalytic activity in the extreme conditions, extremozymes have found the potential biotechnological applications for industrial purposes. A robust extracellular protease activity was detected in the culture broth of , an extreme halophilic bacterium, after a 48 h-incubation. The effect of different media ingredients in a liquid state fermentation was followed with the aim of improving the enzyme production yield. Fractional factorial and Box-Behnken designs were applied to get a 3.4 fold (from 6.0 to 20.3 U mL) improvement of protease production. The distinguishing features of this enzyme were stability at a wide range of pH (5.0-11.0) and temperature (25-60 °C), significant compatibility towards organic solvents, metal ions, chemicals, and surfactants, and hydrolysis of a variety of substrates. The properties of this enzyme can be of tremendous help in terms of the halophilic proteolytic extract's industrial applications.
由于极端酶在极端条件下具有卓越的催化活性，它们已在工业用途中发现了潜在的生物技术应用。在一株极端嗜盐细菌经48小时培养后的培养液中检测到了强大的胞外蛋白酶活性。为提高酶的产量，研究了液态发酵中不同培养基成分的影响。采用分数析因设计和Box-Behnken设计使蛋白酶产量提高了3.4倍（从6.0单位/毫升提高到20.3单位/毫升）。该酶的显著特点是在广泛的pH范围（5.0 - 11.0）和温度范围（25 - 60℃）内稳定，对有机溶剂、金属离子、化学物质和表面活性剂具有显著的兼容性，并且能水解多种底物。就嗜盐蛋白水解提取物的工业应用而言，该酶的特性可能会有极大帮助。
