Key Laboratory of Animal Biotechnology, College of Animal Science and Technology, Northwest A&F University, Xianyang, Shaanxi 712100, P.R. China.
Mol Med Rep. 2019 Feb;19(2):959-966. doi: 10.3892/mmr.2018.9740. Epub 2018 Dec 11.
Adipose tissue has a primary role in lipid and glucose metabolism as a storage site for fatty acids, and also functions as an endocrine organ, producing large numbers of hormones and cytokines. Adipose dysfunction triggers a number of obesity‑associated health problems. The aim of the present study was, therefore, to investigate the molecular mechanisms of white adipose tissue (WAT). The GSE9954 microarray data were downloaded from the Gene Expression Omnibus. Adipose‑specific genes were identified through limma package analysis, based on samples of WAT and 17 other types of non‑adipose tissue obtained from mice. Process and pathway enrichment analyses were performed for these genes. Finally, protein‑protein interaction (PPI) and co‑expression networks were constructed and analyzed. In total, 202 adipose‑specific genes were identified, which were involved in key biological processes (including fat cell differentiation and lipid metabolic process) and one key pathway [namely, the adenine monophosphate‑activated protein kinase (AMPK) signaling pathway]. Construction of the PPI network and further molecular complex detection revealed the presence of 17 key genes, including acetyl‑CoA carboxylase α, peroxisome proliferator‑activated receptor (PPAR) γ and leptin, that were involved in the AMPK, PPAR and insulin signaling pathways. In addition, amine oxidase copper containing 3 and adrenoceptor beta 3 were communication hubs in the co‑expression network of adipose‑specific genes. In conclusion, the present study promotes our understanding of the underlying molecular mechanisms of WAT, and may offer an insight into the prevention and treatment of obesity‑associated diseases caused by adipose dysfunction.
脂肪组织在脂质和葡萄糖代谢中起着主要作用，作为脂肪酸的储存场所，并且还作为内分泌器官，产生大量的激素和细胞因子。脂肪组织功能障碍会引发许多与肥胖相关的健康问题。因此，本研究旨在研究白色脂肪组织（WAT）的分子机制。从基因表达综合数据库中下载 GSE9954 微阵列数据。根据从小鼠获得的 WAT 和 17 种其他非脂肪组织样本，通过 limma 软件包分析鉴定脂肪组织特异性基因。对这些基因进行过程和途径富集分析。最后，构建和分析蛋白质-蛋白质相互作用（PPI）和共表达网络。总共鉴定出 202 个脂肪组织特异性基因，这些基因参与关键的生物学过程（包括脂肪细胞分化和脂质代谢过程）和一个关键途径[即腺苷一磷酸激活蛋白激酶（AMPK）信号通路]。构建 PPI 网络并进一步进行分子复合物检测，揭示了 17 个关键基因的存在，包括乙酰辅酶 A 羧化酶 α、过氧化物酶体增殖物激活受体（PPAR）γ 和瘦素，它们参与 AMPK、PPAR 和胰岛素信号通路。此外，胺氧化酶铜 3 和肾上腺素能受体β 3 是脂肪组织特异性基因共表达网络中的通讯枢纽。总之，本研究促进了我们对 WAT 潜在分子机制的理解，并可能为预防和治疗由脂肪组织功能障碍引起的肥胖相关疾病提供新的思路。
