Department of Nuclear Medicine, The Third Xiangya Hospital, Central South University, Changsha, China.
Department of Radiology, The Third Xiangya Hospital, Central South University, Changsha, China.
Neoplasma. 2019 Mar 5;66(2):245-251. doi: 10.4149/neo_2018_180625N414. Epub 2018 Dec 12.
Long non-coding RNAs (lncRNAs) are strongly associated with cancer biology. The objective of this study is to investigate the expression profile of lncRNAs in human papillary thyroid carcinoma (PTC), and to understand the biological role of lncRNAs and their involvement in PTC oncogenesis. The lncRNAs and messenger RNAs (mRNAs) expression in human PTC and paired adjacent non-cancerous thyroid (NCT) tissues were studied by micro-array, and quantitative real-time polymerase chain reaction (qRT-PCR) validated five differentially expressed lncRNAs. We identified 2,925 significantly differentially expressed lncRNAs with potential roles in PTC, (absolute fold change >2.0; p<0.05). Of these, 1,922 were up-regulated and 933 down-regulated and the qRT-PCR results agreed with micro-array results. Gene ontology (GO) enrichment and pathway analysis then investigated gene function and identified significantly enriched pathways in differentially expressed mRNA's. Many of these pathways were related to cancer, including 60 genes associated with "pathways in cancer" and 34 linked to "proteoglycans in cancer". Co-expression network and target prediction analysis of lncRNAs revealed that TCONS_00020457 can have important roles in PTC. In conclusion, the results of this study indicate that lncRNAs can be important regulators in PTC tumorigenesis and provide understanding of the function and mechanism of lncRNAs related to human papillary thyroid carcinoma.
长链非编码 RNA（lncRNAs）与癌症生物学密切相关。本研究旨在探讨 lncRNAs 在人甲状腺乳头状癌（PTC）中的表达谱，了解 lncRNAs 的生物学功能及其在 PTC 发生中的作用。通过微阵列分析研究了人 PTC 和配对的相邻非癌性甲状腺（NCT）组织中的 lncRNAs 和信使 RNA（mRNA）表达，并通过定量实时聚合酶链反应（qRT-PCR）验证了 5 个差异表达的 lncRNAs。我们鉴定了 2925 个具有 PTC 潜在作用的显著差异表达的 lncRNAs（绝对倍数变化>2.0；p<0.05）。其中，1922 个上调，933 个下调，qRT-PCR 结果与微阵列结果一致。然后进行了基因本体论（GO）富集和途径分析，以研究差异表达 mRNA 的基因功能，并确定了显著富集的途径。这些途径中的许多与癌症有关，包括与“癌症途径”相关的 60 个基因和与“癌症中的蛋白聚糖”相关的 34 个基因。lncRNAs 的共表达网络和靶标预测分析表明，TCONS_00020457 在 PTC 中可能具有重要作用。总之，本研究结果表明，lncRNAs 可能是 PTC 肿瘤发生的重要调节因子，并为理解与人类甲状腺乳头状癌相关的 lncRNAs 的功能和机制提供了依据。
