Jhan Pijush Kanti, Shim Jae-Kyoung, Lee Sukchan, Lee Kyeong-Yeoll
Division of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University, Daegu, Republic of Korea.
Department of Entomology, Patuakhali Science and Technology University, Patuakhali, Bangladesh.
Arch Insect Biochem Physiol. 2019 Feb;100(2):e21503. doi: 10.1002/arch.21517. Epub 2018 Dec 20.
In transmitting plant viruses, insect vectors undergo physiological and behavioral alterations. The whitefly Bemisia tabaci is a vector of tomato yellow leaf curl virus (TYLCV), causing severe damages to various horticultural crop plants. To determine whether whitefly alteration is specific to vector species, the responses to TYLCV ingestion were compared between B. tabaci and Trialeurodes vaporariorum, a nonvector for TYLCV. The two species were reared on TYLCV-infected and noninfected tomato, a host of TYLCV, and their longevity and fecundity were determined while rearing in either tomato or eggplant, a nonhost of TYLCV. TYLCV-ingested B. tabaci increased their developmental rates but reduced fecundity when they were reared in either tomato or eggplant compared with those of TYLCV-free ones. In contrast, TYLCV-ingested T. vaporariorum did not show any of the aforementioned changes when reared on both plant species. In addition, TYLCV-ingested B. tabaci increased their levels of three heat shock protein genes ( hsp20, hsp70, and hsp90) against thermal stress, whereas TYLCV-ingested T. vaporariorum did not. The presence of TYLCV virions was identified in two colonies of both species via polymerase chain reaction analysis. TYLCV was detected in the whole body, saliva, and eggs of B. tabaci, while TYLCV was detected only in the whole body but not in the saliva and eggs of T. vaporariorum. The present results strongly indicated that TYLCV specifically manipulate physiological processes of the vector species, B. tabaci.
在传播植物病毒时，昆虫媒介会发生生理和行为变化。烟粉虱是番茄黄化曲叶病毒（TYLCV）的传播媒介，会对各种园艺作物造成严重损害。为了确定粉虱的变化是否特定于媒介物种，对烟粉虱和温室粉虱（TYLCV的非传播媒介）对摄入TYLCV的反应进行了比较。这两个物种在感染TYLCV和未感染TYLCV的番茄（TYLCV的寄主）上饲养，并在番茄或茄子（TYLCV的非寄主）中饲养时测定它们的寿命和繁殖力。与未摄入TYLCV的烟粉虱相比，摄入TYLCV的烟粉虱在番茄或茄子中饲养时发育速度加快，但繁殖力降低。相比之下，摄入TYLCV的温室粉虱在这两种植物上饲养时均未表现出上述任何变化。此外，摄入TYLCV的烟粉虱针对热应激增加了三种热休克蛋白基因（hsp20、hsp70和hsp90）的水平，而摄入TYLCV的温室粉虱则没有。通过聚合酶链反应分析在这两个物种的两个群体中鉴定出TYLCV病毒粒子的存在。在烟粉虱的全身、唾液和卵中检测到TYLCV，而在温室粉虱中仅在全身检测到TYLCV，在唾液和卵中未检测到。目前的结果有力地表明，TYLCV特异性地操纵了媒介物种烟粉虱的生理过程。
