补体在储存红细胞上的沉积及IgG结合与储存时间无关。
Complement Deposition and IgG Binding on Stored Red Blood Cells Are Independent of Storage Time.
作者信息
Thielen Astrid J F, Meulenbroek Elisabeth M, Baas Inge, Bruggen Robin, Zeerleder Sacha S, Wouters Diana
机构信息
Department of Immunopathology, Sanquin Research, Amsterdam, the Netherlands.
出版信息
Transfus Med Hemother. 2018 Nov;45(6):378-384. doi: 10.1159/000486759. Epub 2018 Mar 9.
BACKGROUND
In the Netherlands, red blood cells (RBCs) are allowed to be stored up to 35 days at 2-6 °C in saline-adenine-glucose-mannitol (SAGM). During storage, RBCs undergo several changes that are collectively known as storage lesion. We investigated to what extent complement deposition and antibody binding occurred during RBC storage and investigated phagocytic uptake in vitro.
METHODS
RBCs were stored for different lengths of time at 2-6 °C in SAGM. Complement deposition and antibody binding were assessed upon storage and after incubation with serum. M1- and M2-type macrophages were generated from blood monocytes to investigate RBC phagocytosis.
RESULTS
No complement deposition was directly observed on stored RBCs, while incubation of RBCs with serum resulted in variable donor-dependent C3 deposition and IgG binding, both independent of storage time. Only 1-4% phagocytosis of stored RBCs by macrophages was observed.
CONCLUSION
RBCs are susceptible to complement deposition and antibody binding independent of storage time. Limited phagocytic uptake by macrophages was observed in vitro.
背景
在荷兰，红细胞（RBCs）在含有生理盐水、腺嘌呤、葡萄糖和甘露醇（SAGM）的保存液中于2 - 6°C下可保存长达35天。在储存过程中，红细胞会发生多种变化，这些变化统称为储存损伤。我们研究了红细胞储存期间补体沉积和抗体结合的发生程度，并在体外研究了吞噬摄取情况。
方法
红细胞在SAGM中于2 - 6°C保存不同时长。在储存时以及与血清孵育后评估补体沉积和抗体结合情况。从血液单核细胞生成M1型和M2型巨噬细胞以研究红细胞吞噬作用。
结果
在储存的红细胞上未直接观察到补体沉积，而红细胞与血清孵育导致不同供体依赖的C3沉积和IgG结合，两者均与储存时间无关。巨噬细胞对储存红细胞的吞噬作用仅观察到1 - 4%。
结论
红细胞易发生补体沉积和抗体结合，且与储存时间无关。在体外观察到巨噬细胞的吞噬摄取有限。
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