Mohammadi Mohammad, Asghari Najmeh, Hashemipoor Mahtab, Borji Shima, Torabi Molouk
Assistant Professor, Department of Periodontics, School of Dentistry, Kerman University of Medical Sciences, Kerman, Iran.
MSc Student, Department of Periodontics, School of Dentistry, Kerman University of Medical Sciences, Kerman, Iran.
Addict Health. 2017 Fall;9(4):222-228.
Although evidence indicates that tobacco use is one of the risk factors for oral cancer, the relationship between opium addiction and oral cancer has not yet been evaluated. The present study was performed aiming to evaluate P53 and epidermal growth factor receptor (EGFR) expression in the gingival tissue of opium-dependent patients.
102 individuals (70 men and 32 women) were entered in the study. 63 and 39 individuals were included in the opium-dependent group and opium-independent group (control group), respectively. 1 gingival biopsy was taken from each individual. The biopsies were fixed in 10% buffered formaldehyde solution and embedded in paraffin at 56 °C. The slides were then stained with Hematoxylin-Eosin (H & E) and Immunohistochemistry (IHC) evaluation was performed with the antihuman antibodies of P53 and EGFR. The protein expression level was later assessed and data were analyzed statistically.
P53 expression was higher among the opium-dependent group, however the difference was not statistically significant (P = 0.052). EGFR expression was significantly higher among the opium-dependent group compared to the control group (P = 0.006).
Opium dependency significantly affects EGFR expression in gingival tissue, however it seems to have no significant effect on P53 expression.
尽管有证据表明吸烟是口腔癌的危险因素之一，但鸦片成瘾与口腔癌之间的关系尚未得到评估。本研究旨在评估鸦片依赖患者牙龈组织中P53和表皮生长因子受体（EGFR）的表达。
102名个体（70名男性和32名女性）参与了本研究。鸦片依赖组和非鸦片依赖组（对照组）分别纳入63人和39人。从每个个体采集1份牙龈活检组织。活检组织用10%缓冲甲醛溶液固定，并在56℃下石蜡包埋。然后将玻片用苏木精-伊红（H&E）染色，并用P53和EGFR的抗人抗体进行免疫组织化学（IHC）评估。随后评估蛋白质表达水平并对数据进行统计学分析。
鸦片依赖组中P53表达较高，但差异无统计学意义（P = 0.052）。与对照组相比，鸦片依赖组中EGFR表达显著更高（P = 0.006）。
鸦片依赖显著影响牙龈组织中EGFR的表达，但似乎对P53表达无显著影响。
