Rong Chao, Liu Dongpo, Li Yan, Yang Kai, Han Xiaobo, Yu Jianwei, Pan Bolun, Zhang Jinsong, Yang Min
Shenzhen Water Affairs (Group) Co., Ltd., Water Building, 1019 Shennan Middle Road, Futian District, Shenzhen, Guangdong China.
2Harbin Institute of Technology Shenzhen Graduate School, HIT Campus of University Town, Nanshan District, Shenzhen, Guangdong China.
Environ Sci Eur. 2018;30(1):45. doi: 10.1186/s12302-018-0175-8. Epub 2018 Nov 27.
Identifying typical odor-causing compounds is essential for odor problem control in drinking water. In this study, aiming at a major water source reservoir in hot and humid areas in southern China, which encountered seasonable odor problems in recent years, an integrated approach including comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry (GC × GC-TOFMS), flavor profile analysis (FPA) and quantitative real-time polymerase chain reaction (qPCR) was adopted to investigate the odor occurrence.
The results indicated that earthy-musty odor is blamed to the seasonable odor problems, and it is consistent with the complaints results from consumers. Fifty-four typical odor compounds were investigated in the reservoir and twelve were detected, of which, 2-methylisoborneol (2-MIB) was significantly increased during the odor event. sp. is the dominant species in the reservoir, which can be further represented by the number of gene with qPCR method ( = 0.746, < 0.001). Oxygen consumption (COD) and dissolved organic carbon (DOC) have great influence on growth of sp., and the release of 2-MIB from the sp. cells is affected by temperature and light.
Our findings demonstrated that 2-MIB is the odor-caused substance in the reservoir and sp. is the main 2-MIB producer, which was confirmed as a benthic filamentous algae. Due to COD and DOC have great influence on sp. growth, further measures to reduce the CODMn and DOC input should be performed. We also demonstrated that the 2-MIB release is affected by temperature and light. The risk of sudden increase of 2-MIB will be reduced by raising the depth of water in the reservoir. Our study will improve the understanding of T&O problems in this city, as well as in other hot and humid area.
识别典型的致臭化合物对于控制饮用水中的气味问题至关重要。本研究针对中国南方炎热潮湿地区的一个主要水源水库,该水库近年来遇到季节性气味问题,采用了包括全二维气相色谱-飞行时间质谱联用(GC×GC-TOFMS)、风味轮廓分析(FPA)和定量实时聚合酶链反应(qPCR)在内的综合方法来调查气味的发生情况。
结果表明,土霉味是导致季节性气味问题的原因,这与消费者的投诉结果一致。在该水库中调查了54种典型的气味化合物,检测到12种,其中2-甲基异冰片醇(2-MIB)在气味事件期间显著增加。某物种是水库中的优势物种,通过qPCR方法可以用该物种的基因数量进一步表示(相关系数=0.746,P<0.001)。耗氧量(COD)和溶解有机碳(DOC)对该物种的生长有很大影响,并且该物种细胞中2-MIB的释放受温度和光照影响。
我们的研究结果表明,2-MIB是水库中的致臭物质,该物种是主要的2-MIB生产者,经证实为底栖丝状藻类。由于COD和DOC对该物种的生长有很大影响,应采取进一步措施减少化学需氧量和DOC的输入。我们还证明了2-MIB的释放受温度和光照影响。通过提高水库的水深可以降低2-MIB突然增加的风险。我们的研究将增进对该城市以及其他炎热潮湿地区气味和味道问题的理解。