Venkatesan M M, Buysse J M, Kopecko D J
Division of Communicable Diseases and Immunology, Walter Reed Army Institute of Research, Washington, DC 20307-5100.
Proc Natl Acad Sci U S A. 1988 Dec;85(23):9317-21. doi: 10.1073/pnas.85.23.9317.
The large invasion plasmid of Shigella flexneri M9OT-W was used to generate recombinant plasmids carrying the ipaA, -B, -C, and -D genes, whose products are associated with the entry of the bacteria into colonic epithelial cells. Complete DNA sequences of ipaB, -C, and -D were determined. The proteins predicted (62, 42, and 37 kDa, respectively) from the nucleotide sequences lack a signal-peptide sequence. Hydrophilic segments of the IpaB and IpaC proteins were found to overlap known epitopic domains of these membrane antigens. Analysis of total RNA demonstrated that temperature control of ipa gene expression occurs at the level of transcription. Multiple mRNA bands were detected by using ipa gene fragments as hybridization probes, and a putative transcript map for the ipa genes was constructed. Comparison of this map with the DNA sequence reveals a complex system of ipa gene regulation.
弗氏志贺菌M9OT-W的大型侵袭质粒被用于构建携带ipaA、-B、-C和-D基因的重组质粒，这些基因的产物与细菌进入结肠上皮细胞的过程相关。测定了ipaB、-C和-D的完整DNA序列。从核苷酸序列预测的蛋白质（分别为62、42和37 kDa）缺乏信号肽序列。发现IpaB和IpaC蛋白的亲水区与这些膜抗原的已知表位域重叠。总RNA分析表明，ipa基因表达的温度控制发生在转录水平。使用ipa基因片段作为杂交探针检测到多个mRNA条带，并构建了ipa基因的推定转录图谱。将该图谱与DNA序列进行比较，揭示了一个复杂的ipa基因调控系统。
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