与鼠疫耶尔森菌低钙反应相关的温度控制质粒调控子
Temperature-controlled plasmid regulon associated with low calcium response in Yersinia pestis.
作者信息
Yother J, Chamness T W, Goguen J D
出版信息
J Bacteriol. 1986 Feb;165(2):443-7. doi: 10.1128/jb.165.2.443-447.1986.
Abstract
Both the low calcium response and virulence in Yersinia pestis strain KIM5 are mediated by genes located on the 75.4-kilobase plasmid pCD1. The results presented here demonstrate the existence of two new genetic loci of pCD1 whose expression is regulated in response to temperature. Levels of transcription in the trtA and trtB loci were elevated 12- to 16-fold at 37 degrees C compared with levels of transcription at 30 degrees C. In addition, the absolute levels of transcription were the highest that have been reported for genes on pCD1. Mutations in trtB also abolished production of the V antigen. Thermal induction at these loci was dramatically reduced in strains harboring a Tn5 insertion in the lcrF locus of pCD1. lcrF lies 33 and 13 kilobases from the trtA and trtB loci, respectively. Thus, lcrF is a positive regulatory gene responsible for temperature-induced transcription of genes required for the low calcium response.
摘要
鼠疫杆菌KIM5株的低钙反应和毒力均由位于75.4千碱基的质粒pCD1上的基因介导。本文给出的结果证明了pCD1上存在两个新的遗传位点，其表达受温度调节。与30℃时的转录水平相比，trtA和trtB位点在37℃时的转录水平提高了12至16倍。此外，转录的绝对水平是pCD1上基因所报道的最高水平。trtB中的突变也消除了V抗原的产生。在pCD1的lcrF位点插入Tn5的菌株中，这些位点的热诱导显著降低。lcrF分别位于距trtA和trtB位点33和13千碱基处。因此，lcrF是一个正向调节基因，负责低钙反应所需基因的温度诱导转录。
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