Department of Biology, Saint Mary's University, 923 Robie Street, Halifax, Nova Scotia B3H 3C3, Canada.
Department of Biology, Saint Mary's University, 923 Robie Street, Halifax, Nova Scotia B3H 3C3, Canada; Department of Biology, Mount Saint Vincent University, 166 Bedford Highway, Halifax, Nova Scotia B3M 2J6, Canada.
J Plant Physiol. 2019 Feb;233:12-19. doi: 10.1016/j.jplph.2018.12.006. Epub 2018 Dec 12.
It is now well documented that plants produce methane (CH) under aerobic conditions. However, the nature of methane production in plants and all the potential precursors and environmental factors that can be involved in the process are not fully understood. Earlier studies have suggested several chemical compounds, including the amino acid methionine, as precursors of aerobic methane in plants, but none have explored other amino acids as potential precursors or blue light as a driving force of methane emission. We examined the effects of blue light, and the promoter or inhibitor of endogenous ethylene on methane and ethylene emissions, amino acids, and some plant physiological parameters in canola (Brassica napus). Plants were grown under four light conditions: no supplemental blue light, and low, medium, or high blue light, and exposed to three chemical treatments: no chemical application, ethylene promoter (kinetin), or ethylene inhibitor (silver nitrate). Regardless of chemical treatment, blue light significantly increased methane emission, which was accompanied by decreased plant biomass, gas exchange, and flavonoids, but by increased wax, and most amino acids. This study revealed that blue light drives aerobic methane emission from plants by releasing of methyl group from a number of amino acids, and that the methane production in plants may have several pathways.
现在已有大量文献证明，植物在有氧条件下会产生甲烷（CH）。然而，植物中产甲烷的本质以及所有可能涉及该过程的潜在前体和环境因素尚未完全被理解。早期研究表明，包括氨基酸蛋氨酸在内的几种化学化合物是植物有氧产甲烷的前体，但尚未有研究探索其他氨基酸是否也可作为潜在前体，或蓝光是否可作为甲烷排放的驱动力。我们研究了蓝光以及内源性乙烯的促进剂或抑制剂对油菜（甘蓝型油菜）中甲烷和乙烯排放、氨基酸和一些植物生理参数的影响。植物在四种光照条件下生长：无补充蓝光，低、中或高光蓝，以及暴露于三种化学处理下：无化学处理、乙烯促进剂（激动素）或乙烯抑制剂（硝酸银）。无论化学处理如何，蓝光均显著增加了甲烷排放，同时伴随着植物生物量、气体交换和类黄酮的减少，而蜡质和大多数氨基酸的增加。本研究表明，蓝光通过从多种氨基酸中释放甲基基团来驱动植物的有氧甲烷排放，并且植物中产甲烷可能存在多条途径。
