Gibadulin R A, Iuferov V P, Lideman L F, Parasiuk N A, Ionova K S
Vopr Virusol. 1988 Jul-Aug;33(4):428-31.
The paper describes a method using plasmid construction pSC11 for generation of recombinant vaccinia viruses supporting coexpression of heterologous genes and beta-galactosidase. The Ca2+-phosphate method of cell transfection by recombinant DNAs generated on the basis of pSC11, and selection of recombinant viruses from blue plaques of virus-infected cells in the presence of X-gala are reported at length.
