Tabecka-Lonczynska Anna, Mytych Jennifer, Solek Przemyslaw, Kowalewski Mariusz P, Koziorowski Marek
Department of Animal Physiology and Reproduction, Faculty of Biotechnology, University of Rzeszow, Werynia 502, 36-100, Kolbuszowa, Poland.
Department of Animal Physiology and Reproduction, Faculty of Biotechnology, University of Rzeszow, Werynia 502, 36-100, Kolbuszowa, Poland.
Theriogenology. 2019 Mar 1;126:199-205. doi: 10.1016/j.theriogenology.2018.12.016. Epub 2018 Dec 5.
The European bisons are the largest mammals of Europe that are still in danger of extinction. The species conservation is associated with their continuous reproduction, and bisons are characterized by the well-pronounced seasonality of reproductive processes. However, the exact mechanisms regulating their reproduction still remain unknown. Our previous studies indicated the involvement of some of the growth factors in the regulation of male seasonal reproductive activities in bison, showing expression patterns that seemed to be regulated by the length of the daylight. In the present study, using RT-PCR and Western blot approaches, we verified the expression and possible relationship between the insulin-like growth factor (IGF-1), its receptor (IGF-1R), and klotho in testis and epididymis of the European bison in pre- and post-reproductive periods, i.e., in June and in December. The observed expression of IGF-1 and IGF-1R mRNA in testis and epididymis was higher in June than in December. At the same time, klotho mRNA expression in both testis and epididymis did not differ between the analyzed seasons. However, along with the higher levels of IGF-1R protein observed in June, klotho protein levels for the membrane form and for the secrete form were higher in December than in June. Finally, the messenger and protein expression profiles presented herein indicate the importance of both the IGF-system and klotho in reproductive processes in the European bison, implying their involvement in the regulation of seasonal testicular activity in males of this threatened species.
欧洲野牛是欧洲现存仍面临灭绝危险的最大哺乳动物。物种保护与其持续繁殖相关，而野牛的生殖过程具有明显的季节性。然而，调节其繁殖的确切机制仍不清楚。我们之前的研究表明，一些生长因子参与了野牛雄性季节性生殖活动的调节，其表达模式似乎受日照长度的调控。在本研究中，我们采用逆转录聚合酶链反应（RT-PCR）和蛋白质免疫印迹法，验证了胰岛素样生长因子（IGF-1）、其受体（IGF-1R）和klotho蛋白在欧洲野牛繁殖前期和繁殖后期（即6月和12月）睾丸和附睾中的表达及可能存在的关系。观察到睾丸和附睾中IGF-1和IGF-1R mRNA的表达在6月高于12月。同时，睾丸和附睾中klotho mRNA的表达在各分析季节间无差异。然而，尽管6月观察到较高水平的IGF-1R蛋白，但12月膜形式和分泌形式的klotho蛋白水平均高于6月。最后，本文呈现的信使核糖核酸和蛋白质表达谱表明，IGF系统和klotho蛋白在欧洲野牛的生殖过程中均具有重要作用，这意味着它们参与了这种濒危物种雄性季节性睾丸活动的调节。
