中国舌鳎（Cynoglossus semilaevis）Lhx9 的克隆、组织分布和甲基化分析。
Cloning, tissue distribution and methylation analyses of Lhx9 in Chinese tongue sole (Cynoglossus semilaevis).
机构信息
Yellow Sea Fisheries Research Institute, CAFS, Key Lab for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Qingdao 266071, China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China.
Yellow Sea Fisheries Research Institute, CAFS, Key Lab for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Qingdao 266071, China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China.
出版信息
Gene. 2019 Apr 5;691:176-184. doi: 10.1016/j.gene.2018.12.025. Epub 2018 Dec 21.
Lhx9 is a LIM-homeodomain protein related to gonad development and sex reversal. In this study, we cloned and characterized CS-Lhx9 in the gonads of the Chinese tongue sole (Cynoglossus semilaevis). The full-length cDNA of CS-Lhx9 was 3123 bp, including an ORF of 1149 bp encoding 383 amino acids which contains two LIM domains and a homeobox domain. CS-Lhx9 transcripts were primarily observed in the testis of male and neomale at 1 yah, but nearly undetectable in the ovary. During the development of gonad, CS-Lhx9 exhibited an increasing trend and appeared to reach its peak value of expression in testis at 1 yah. In situ hybridization was performed in male and neomale gonads at 210 dah and 1 yah. The results showed strong CS-Lhx9 signals in the spermatids and spermatozoa (germ cells). Methylation level of CS-Lhx9 promoter was higher in female and lower in male and neomale gonads, showing a negative correlation with CS-Lhx9 expression.
Lhx9 是一种与性腺发育和性别逆转有关的 LIM 同源域蛋白。在这项研究中，我们在黄唇鱼（Cynoglossus semilaevis）的性腺中克隆并鉴定了 CS-Lhx9。CS-Lhx9 的全长 cDNA 为 3123bp，包含一个 1149bp 的 ORF，编码 383 个氨基酸，其中包含两个 LIM 结构域和一个同源盒结构域。CS-Lhx9 转录本主要在 1 月龄雄性和拟雄性的睾丸中观察到，但在卵巢中几乎无法检测到。在性腺发育过程中，CS-Lhx9 呈现出增加的趋势，在 1 月龄的睾丸中似乎达到了表达高峰。在 210 日龄和 1 月龄的雄性和拟雄性性腺中进行了原位杂交。结果显示，CS-Lhx9 信号在精母细胞和精子（生殖细胞）中较强。CS-Lhx9 启动子的甲基化水平在雌性和拟雄性性腺中较高，在雄性性腺中较低，与 CS-Lhx9 的表达呈负相关。
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