Yellow Sea Fisheries Research Institute, CAFS, Key Lab for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Qingdao, 266071, China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China.
Yellow Sea Fisheries Research Institute, CAFS, Key Lab for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Qingdao, 266071, China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China.
Theriogenology. 2019 Oct 15;138:1-8. doi: 10.1016/j.theriogenology.2019.06.035. Epub 2019 Jun 20.
Dmrt2 is a member of the dmrt gene family with a conserved zinc finger-like DNA-binding motif (DM domain). In the present study, CS-dmrt2 was cloned from the gonads of Chinese tongue sole (Cynoglossus semilaevis). The full-length cDNA of CS-dmrt2 is 2834 bp in length, with a 251 bp 5'-untranslated region (UTR), a 1086 bp 3'-UTR and a 1503 bp open reading frame (ORF) that encodes a 501-amino-acid peptide. qPCR revealed that CS-dmrt2 was mainly expressed in C. semilaevis testes. In situ hybridization (ISH) showed CS-dmrt2 expression throughout early gonadal development (36 days after hatching (dah) and 86 dah), but the expression was higher in male gonads than in female gonads. CS-dmrt2 mRNA was highly expressed in male germ cells. Comparison of methylation levels between females and males demonstrated hypo-methylated levels of the CS-dmrt2 promoter in the male gonads, which is consistent with the high mRNA expression. These results suggest that the CS-dmrt2 gene may play a functional role in gonadal differentiation/development and germ cell maturation in the testis.
DMRT2 是 DMRT 基因家族的成员，具有保守的锌指样 DNA 结合基序（DM 结构域）。本研究从中国牙鲆（Cynoglossus semilaevis）的性腺中克隆了 CS-dmrt2。CS-dmrt2 的全长 cDNA 长 2834 bp，含有 251 bp 的 5'-非翻译区（UTR）、1086 bp 的 3'-UTR 和 1503 bp 的开放阅读框（ORF），编码 501 个氨基酸的肽。qPCR 显示 CS-dmrt2 主要在 C. semilaevis 睾丸中表达。原位杂交（ISH）显示 CS-dmrt2 在早期性腺发育过程中（孵化后 36 天（dah）和 86 dah）广泛表达，但在雄性性腺中的表达高于雌性性腺。CS-dmrt2 mRNA 在雄性生殖细胞中高度表达。雌性和雄性之间的甲基化水平比较表明，雄性性腺中 CS-dmrt2 启动子的甲基化水平较低，与高 mRNA 表达一致。这些结果表明，CS-dmrt2 基因可能在睾丸分化/发育和精子细胞成熟过程中发挥功能作用。
