Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China; College of Forestry, Nanjing Forestry University, Nanjing, China.
Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China; College of Forestry, Nanjing Forestry University, Nanjing, China.
Comp Biochem Physiol Part D Genomics Proteomics. 2019 Mar;29:211-220. doi: 10.1016/j.cbd.2018.12.002. Epub 2018 Dec 11.
Clostera restitura Walker (Lepidoptera: Notodontidae) is one of the most destructive defoliators of poplars in China. We constructed an antennal transcriptome using Illumina Hiseq 2500™ sequencing and characterized the expression profiles of odorant binding proteins for better understanding of the olfactory receptive system and the role of putative olfactory proteins in C. restitura. A total of 165 transcripts were identified, including 43 transcripts encoding putative odorant-binding proteins (OBPs), 13 chemosensory proteins (CSPs), 78 odorant receptors (ORs), 15 ionotropic receptors (IRs), 13 gustatory receptors (GRs), and 3 sensory neuron membrane proteins (SNMPs). Furthermore, we systematically analyzed expression patterns of eight OBPs from different tissues of both C. restitura sexes by using reverse transcription PCR and quantitative real time PCR (RT-qPCR). The expression level of CresGOBP2 in female antennae was approximately two times higher than in males, and two pheromone binding proteins PBPs (CresPBP1 and -PBP3) and three OBPs (CresOBP9, -10, and -16) were more highly enriched in male antennae than in female antennae. CresOBP10 showed a remarkably high expression in legs compared to other studied insects. Our results suggested that these proteins might play a key role in foraging, seeking mates, and host recognition in C. restitura. Our findings provided a foundation for future studies on the molecular mechanisms controlling the olfactory system in C. restitura and potential novel targets for pest control strategies.
云杉窄吉丁（Clostera restitura Walker）（鳞翅目：Notodontidae）是中国杨树最具破坏性的食叶害虫之一。我们使用 Illumina Hiseq 2500™测序构建了一个触角转录组，并对气味结合蛋白的表达谱进行了特征描述，以便更好地了解嗅觉感受系统和假定嗅觉蛋白在 C. restitura 中的作用。共鉴定出 165 个转录本，包括 43 个编码假定气味结合蛋白（OBPs）、13 个化学感觉蛋白（CSPs）、78 个气味受体（ORs）、15 个离子型受体（IRs）、13 个味觉受体（GRs）和 3 个感觉神经元膜蛋白（SNMPs）。此外，我们通过反转录 PCR 和定量实时 PCR（RT-qPCR）系统地分析了来自不同组织的 8 种 OBPs 的表达模式。雌性触角中 CresGOBP2 的表达水平约为雄性的两倍，两种信息素结合蛋白 PBPs（CresPBP1 和 -PBP3）和三种 OBPs（CresOBP9、-10 和 -16）在雄性触角中的丰度明显高于雌性触角。CresOBP10 在腿部的表达水平明显高于其他研究的昆虫。这些结果表明，这些蛋白可能在 C. restitura 的觅食、寻找配偶和宿主识别中发挥关键作用。我们的研究结果为进一步研究 C. restitura 嗅觉系统的分子机制和潜在的害虫防治策略提供了新的思路。
