Wang Jing Jing, Ma Chao, Yue Yang, Yang Jingfang, Chen Li Xiang, Wang Yi Ting, Zhao Chen Chen, Gao Xuyuan, Chen Hong Song, Ma Wei Hua, Zhou Zhongshi
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
National Nanfan Research Institute, Chinese Academy of Agricultural Sciences, Sanya, China.
Front Physiol. 2024 Feb 19;15:1354530. doi: 10.3389/fphys.2024.1354530. eCollection 2024.
The melon fly, (Coquillett) (Tephritidae: Diptera), is an invasive pest that poses a significant threat to agriculture in Africa and other regions. Flies are known to use their olfactory systems to recognise environmental chemical cues. However, the molecular components of the chemosensory system of are poorly characterised. To address this knowledge gap, we have used next-generation sequencing to analyse the antenna transcriptomes of sexually immature adults. The results have identified 160 potential chemosensory genes, including 35 odourant-binding proteins (OBPs), one chemosensory protein (CSP), three sensory neuron membrane proteins (SNMPs), 70 odourant receptors (ORs), 30 ionotropic receptors (IRs), and 21 gustatory receptors (GRs). Quantitative real-time polymerase chain reaction quantitative polymerase chain reaction was used to validate the results by assessing the expression profiles of 25 ORs and 15 OBPs. Notably, high expression levels for were observed in both the female and male antennae. Furthermore, exhibited biased expression in the male antennae, whereas showed biased expression in the female antennae. This comprehensive investigation provides valuable insights into insect olfaction at the molecular level and will, thus, help to facilitate the development of enhanced pest management strategies in the future.
瓜实蝇（Bactrocera cucurbitae (Coquillett)，实蝇科：双翅目）是一种入侵性害虫，对非洲和其他地区的农业构成重大威胁。已知果蝇利用其嗅觉系统识别环境化学线索。然而，瓜实蝇化学感应系统的分子成分却鲜为人知。为了填补这一知识空白，我们利用下一代测序技术分析了未成熟成虫的触角转录组。结果鉴定出160个潜在的化学感应基因，包括35个气味结合蛋白（OBP）、1个化学感应蛋白（CSP）、3个感觉神经元膜蛋白（SNMP）、70个气味受体（OR）、30个离子otropic受体（IR）和21个味觉受体（GR）。通过定量实时聚合酶链反应（qPCR）评估25个OR和15个OBP的表达谱来验证结果。值得注意的是，在雌性和雄性触角中均观察到[具体基因]的高表达水平。此外，[具体基因]在雄性触角中表现出偏向性表达，而[具体基因]在雌性触角中表现出偏向性表达。这项全面的研究为昆虫嗅觉的分子水平提供了有价值的见解，因此将有助于促进未来增强害虫管理策略的发展。
