比较低聚果糖与β-乳球蛋白的糖化反应及未经与经动态高压微射流处理后的抗原性和构象变化。
Comparison of antigenicity and conformational changes to β-lactoglobulin following kestose glycation reaction with and without dynamic high-pressure microfluidization treatment.
机构信息
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
出版信息
Food Chem. 2019 Apr 25;278:491-496. doi: 10.1016/j.foodchem.2018.11.094. Epub 2018 Nov 22.
Previous work indicated that conformational changes of β-lactoglobulin (β-LG) induced by dynamic high pressure microfluidization (DHPM) was related to the increase of antigenicity. In this study, β-LG glycated with 1-kestose and combined with DHPM decreased the antigenicity of β-LG. The antigenicity of control, β-LG-kestose (0.1 MPa) and β-LG-kestose (80 MPa) were 100, 79 and 42 μg/mL respectively. The molecular weight of β-LG conjugated to kestose increased from 18.4 to 19.6 kDa and its conformation scarcely changed. Conversely, combined with DHPM treatment (80 MPa), β-LG conjugated to kestose formed two conjugates with molecular weight of 18.8 and 19.8 kDa, respectively. Furthermore, the unfolding of β-LG as a result of the treatments is reflected by a decrease of intrinsic and synchronous fluorescence intensity and changes to the secondary structure. The conformational changes induced by DHPM and glycation treatments synergistically decrease the antigenicity of β-LG due to more masked or disrupted epitopes.
先前的研究表明，动态高压微射流（DHPM）诱导的β-乳球蛋白（β-LG）构象变化与抗原性的增加有关。在本研究中，用 1-蔗果三糖糖化的β-LG 并结合 DHPM 降低了β-LG 的抗原性。对照物、β-LG-蔗果三糖（0.1 MPa）和β-LG-蔗果三糖（80 MPa）的抗原性分别为 100、79 和 42 μg/mL。与蔗果三糖结合的β-LG 的分子量从 18.4 增加到 19.6 kDa，其构象几乎没有变化。相反，与 DHPM 处理（80 MPa）结合后，β-LG 与蔗果三糖形成了两种分子量分别为 18.8 和 19.8 kDa 的缀合物。此外，由于更多的表位被掩盖或破坏，β-LG 的展开反映在内在和同步荧光强度的降低以及二级结构的变化上。DHPM 和糖化处理诱导的构象变化协同降低了β-LG 的抗原性。
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