Universidad de Colima, Centro Universitario de Investigaciones Biomédicas, Av. 25 de Julio No. 965, Col. Villas San Sebastián, Colima 28045, Colima, Mexico.
Universidad de Colima-Cátedras-CONACyT, Centro Universitario de Investigaciones Biomédicas, Av. 25 de Julio No. 965, Col. Villas San Sebastián, Colima 28045, Colima, Mexico.
Molecules. 2018 Dec 21;24(1):36. doi: 10.3390/molecules24010036.
Capsaicin is an agonist of the transient receptor potential vanilloid type 1 (TRPV1) channel, which has been related to the pathophysiology of kidney disease secondary to diabetes. This study aimed to evaluate the chronic effect of capsaicin administration on biomarkers of kidney injury in an experimental rat model of diabetes. Male Wistar rats were assigned to four groups: (1) healthy controls without diabetes (CON), (2) healthy controls plus capsaicin at 1 mg/kg/day (CON + CAPS), (3) experimental diabetes without capsaicin (DM), and (4) experimental diabetes plus capsaicin at 1 mg/kg/day (DM + CAPS). For each group, 24-h urine samples were collected to determine diuresis, albumin, cystatin C, β2 microglobulin, epidermal growth factor (EGF), alpha (1)-acid glycoprotein, and neutrophil gelatinase-associated lipocalin (NAG-L). Blood samples were drawn to measure fasting glucose. After 8 weeks, the CON + CAPS and DM + CAPS groups showed increased diuresis compared to the CON and DM groups, but the difference was significant only in the DM + CAPS group. The two-way ANOVA only showed a statistically significant effect of CAPS on the urinary EGF levels, as well as a tendency to have a significant effect in the urinary NAG-L levels. The EGF levels decreased in both CAPS-treated groups, but the change was only significant in the CON + CAPS group vs. CON group; and the NAG-L levels were lower in both CAPS-treated groups. These results show that capsaicin had a diuretic effect in healthy and diabetic rats; additionally, it increased the urinary EGF levels and tended to decrease the urinary NAG-L levels.
辣椒素是瞬时受体电位香草素 1 型 (TRPV1) 通道的激动剂，与糖尿病继发肾病的病理生理学有关。本研究旨在评估辣椒素给药对糖尿病实验大鼠模型肾损伤生物标志物的慢性影响。雄性 Wistar 大鼠被分为四组：（1）无糖尿病的健康对照组（CON），（2）每日给予 1mg/kg 辣椒素的健康对照组（CON+CAPS），（3）无辣椒素的实验性糖尿病组（DM），和（4）每日给予 1mg/kg 辣椒素的实验性糖尿病组（DM+CAPS）。对于每组，收集 24 小时尿液样本以确定尿量、白蛋白、胱抑素 C、β2 微球蛋白、表皮生长因子 (EGF)、α(1)-酸性糖蛋白和中性粒细胞明胶酶相关脂质运载蛋白 (NAG-L)。抽取血液样本以测量空腹血糖。8 周后，CON+CAPS 和 DM+CAPS 组的尿量较 CON 和 DM 组增加，但仅在 DM+CAPS 组差异有统计学意义。双因素方差分析仅显示 CAPS 对尿 EGF 水平有统计学显著影响，并且对尿 NAG-L 水平有显著影响的趋势。两组 CAPS 治疗组的 EGF 水平均降低，但仅在 CON+CAPS 组与 CON 组相比差异有统计学意义；并且两组 CAPS 治疗组的 NAG-L 水平均降低。这些结果表明，辣椒素在健康和糖尿病大鼠中具有利尿作用；此外，它增加了尿 EGF 水平，并倾向于降低尿 NAG-L 水平。
