Nomura Harue, Takahashi Sakiko, Tohara Yuki, Isshiki Yasunori, Sakuda Keisuke, Sakuma Katsuya, Kondo Seiichi
Department of Microbiology, School of Pharmaceutical Sciences, Josai University.
Fragrance R&D Department, Ogawa & Co., Ltd.
Biocontrol Sci. 2018;23(4):241-244. doi: 10.4265/bio.23.241.
Acanthamoeba castellanii, a ubiquitous organism in water environments, is pathogenic toward humans and also is a host for bacteria of the genus Legionella, a causative agent of legionellosis. Fragrance ingredients were investigated for their antibacterial activity against planktonic Legionella pneumophila, amoebicidal activity against A. castellanii, and inhibitory effect against L. pneumophila uptake into A. castellanii. Helional exhibited relatively high antibacterial activity [minimum inhibitory concentration (MIC) , 32.0 μg/mL] . Anis aldehyde, canthoxal, helional and vanillin exhibited amoebicidal activity (IC values, 58.4±2.0, 71.2±14.7, 66.8±8.3 and 49.1±2.5μg/mL, respectively) . L. pneumophila pretreatment with sub-MICs (0.25×MIC) of anis aldehyde, canthoxal, cortex aldehyde 50 percent or vanillin evidently reduced L. pneumophila uptake into A. castellanii (p < 0.01) . Thus, fragrance ingredients were good candidates for disinfectant against L. pneumophila and A. castellanii.
卡氏棘阿米巴是水环境中一种普遍存在的生物，对人类具有致病性，也是嗜肺军团菌属细菌的宿主，嗜肺军团菌是军团病的病原体。研究了香料成分对浮游嗜肺军团菌的抗菌活性、对卡氏棘阿米巴的杀阿米巴活性以及对嗜肺军团菌被卡氏棘阿米巴摄取的抑制作用。铃兰醛表现出相对较高的抗菌活性[最低抑菌浓度（MIC），32.0μg/mL]。茴香醛、樟脑醛、铃兰醛和香草醛表现出杀阿米巴活性（IC值分别为58.4±2.0、71.2±14.7、66.8±8.3和49.1±2.5μg/mL）。用茴香醛、樟脑醛、50%皮层醛或香草醛的亚MICs（0.25×MIC）对嗜肺军团菌进行预处理，显著降低了嗜肺军团菌被卡氏棘阿米巴摄取的量（p<0.01）。因此，香料成分是对抗嗜肺军团菌和卡氏棘阿米巴的良好消毒剂候选物。
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