Wang Lin, Yan Ting, Zhang Kexia, Li Feifeng, Jia Jingming, Hu Gaosheng
School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, Liaoning, China.
Heilongjiang Provincial Institute for Food and Drug Control, Harbin, Heilongjiang, China.
Biomed Chromatogr. 2019 Apr;33(4):e4470. doi: 10.1002/bmc.4470. Epub 2019 Feb 4.
A method based on ultra-performance liquid chromatography-tandem mass spectrometry has been developed for the rapid and simultaneous determination of five catechins and four theaflavins in rat plasma using ethyl gallate as internal standard. The pharmacokinetic profiles of these compounds were compared after oral administration of five kinds of Da Hong Pao tea to rats. Biosamples processed with a mixture of β-glucuronidase and sulfatase were extracted with ethyl acetate-isopropanol. Chromatographic separation was achieved by gradient elution using 10 mm HCOONH solution and methanol as the mobile phase. Analytes were detected using negative ion electrospray ionization in multiple reaction monitoring mode. The lower limits of quantification were 1.0, 0.74 and 0.5 ng/mL for theaflavins, two catechins and three catechins, respectively. The validation parameters were well within acceptable limits. The average half-lives (t ) in blood of the reference solution group was much shorter than those of tea samples. The values of AUC and C of the polyphenols and theaflavins exhibited linear pharmacokinetic characteristics which were related to the dose concentration.
建立了一种基于超高效液相色谱-串联质谱法的方法，以没食子酸乙酯为内标，用于快速同时测定大鼠血浆中的五种儿茶素和四种茶黄素。给大鼠口服五种大红袍茶后，比较了这些化合物的药代动力学特征。用β-葡萄糖醛酸酶和硫酸酯酶混合物处理的生物样品用乙酸乙酯-异丙醇萃取。采用10 mM甲酸铵溶液和甲醇作为流动相进行梯度洗脱实现色谱分离。在多反应监测模式下使用负离子电喷雾电离检测分析物。茶黄素、两种儿茶素和三种儿茶素的定量下限分别为1.0、0.74和0.5 ng/mL。验证参数均在可接受范围内。参比溶液组血液中的平均半衰期（t）比茶样品的平均半衰期短得多。多酚和茶黄素的AUC和C值呈现出与剂量浓度相关的线性药代动力学特征。
