胡桃醌处理的玉米（Zea mays L.）和小麦（Triticum aestivum L.）种子中过氧化氢酶基因的转录谱分析

Transcriptional profiling of catalase genes in juglone-treated seeds of maize (Zea mays L.) and wheat (Triticum aestivum L.).

作者信息

Sytykiewicz Hubert, Kozak Agnieszka, Leszczyński Bogumił, Sempruch Cezary, Łukasik Iwona, Sprawka Iwona, Kmieć Katarzyna, Kurowska Monika, Kopczyńska Aldona, Czerniewicz Paweł

机构信息

1 Department of Biochemistry and Molecular Biology, University of Natural Sciences and Humanities , Prusa 14, 08-110 Siedlce , Poland.

2 Department of Entomology, University of Life Sciences , Leszczyńskiego 7, 20-069 Lublin , Poland.

出版信息

Acta Biol Hung. 2018 Dec;69(4):449-463. doi: 10.1556/018.69.2018.4.7.

DOI:10.1556/018.69.2018.4.7

PMID:30587016

Abstract

The major aim of the present study was to investigate the influence of juglone (JU; 5-hydroxy-1,4-naphthoquinone) treatments on the expression level of Cat1, Cat2 and Cat3 genes, encoding the respective catalase isozymes in maize (Zea mays L.) and wheat (Triticum aestivum L.) seeds. In parallel, germination efficiency, catalase (CAT) activity and hydrogen peroxide (HO) content in juglone-exposed cereal seeds were assessed. Juglone applications significantly stimulated abundance of three target catalase transcripts as well as induced CAT activity and generation of HO in both maize and wheat kernels. Furthermore, germination process of juglone-affected maize seeds was more severe suppressed than in case of wheat kernels. The role of juglone in triggering the oxidative stress as well as antioxidative responses in seeds of the studied model cereal species are discussed.

摘要

本研究的主要目的是探究胡桃醌（JU；5-羟基-1,4-萘醌）处理对玉米（Zea mays L.）和小麦（Triticum aestivum L.）种子中分别编码过氧化氢酶同工酶的Cat1、Cat2和Cat3基因表达水平的影响。同时，评估了经胡桃醌处理的谷物种子的发芽效率、过氧化氢酶（CAT）活性和过氧化氢（HO）含量。施用胡桃醌显著刺激了三种目标过氧化氢酶转录本的丰度，并诱导了玉米和小麦籽粒中的CAT活性及HO的产生。此外，受胡桃醌影响的玉米种子的发芽过程比小麦籽粒受到的抑制更严重。本文讨论了胡桃醌在引发所研究的模式谷物种子中的氧化应激以及抗氧化反应方面的作用。