School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, CMU - Rue Michel Servet, 1, 1211, Geneva 4, Switzerland.
Department of Analytical Chemistry, University of Pannonia, Egyetem u. 10, 8200, Veszprém, Hungary.
J Chromatogr A. 2019 Mar 15;1588:159-162. doi: 10.1016/j.chroma.2018.12.037. Epub 2018 Dec 18.
It was shown earlier that in serially coupled column systems, not only retention (selectivity) but peak width as well can change and the apparent peak width depends on the order of the columns. A generic equation has been developed to describe the apparent efficiency of serially coupled columns in gradient elution liquid chromatography mode. In a previous work, the theoretical basis for a two-column system was developed and by extending and generalizing this model, it is now possible to predict the apparent peak width for the combination of any column segments in any formats (columns possessing various efficiencies and lengths). (Identical retention and selectivity have been assumed to develop this model.) At the end, column order can be a useful parameter to set the desired efficiency of coupled systems.
早前的研究表明，在串联柱系统中，不仅保留（选择性）而且峰宽也会发生变化，并且表观峰宽取决于柱的顺序。已经开发了一个通用方程来描述在梯度洗脱液相色谱模式下串联柱的表观效率。在以前的工作中，开发了两柱系统的理论基础，并通过扩展和推广该模型，现在可以预测任何格式（具有不同效率和长度的柱）的任何柱段组合的表观峰宽。（为了开发该模型，假设保留和选择性相同。）最后，柱顺序可以是一个有用的参数，用于设置耦合系统所需的效率。
