Sacca Paula Alejandra, Mazza Osvaldo Néstor, Scorticati Carlos, Vitagliano Gonzalo, Casas Gabriel, Calvo Juan Carlos
Institute of Biology and Experimental Medicine (IBYME), CONICET, Buenos Aires, Argentina
Department of Urology, School of Medicine, University of Buenos Aires, Clínical Hospital "José de San Martín", Buenos Aires, Argentina.
Cancer Genomics Proteomics. 2019 Jan-Feb;16(1):29-58. doi: 10.21873/cgp.20110.
BACKGROUND/AIM: Periprostatic adipose tissue (PPAT) directs tumour behaviour. Microenvironment secretome provides information related to its biology. This study was performed to identify secreted proteins by PPAT, from both prostate cancer and benign prostate hyperplasia (BPH) patients.
Liquid chromatography-mass spectrometry-based proteomic analysis was performed in PPAT-conditioned media (CM) from patients with prostate cancer (CMs-T) (stage T3: CM-T3, stage T2: CM-T2) or benign disease (CM-BPH).
The highest number and diversity of proteins was identified in CM-T3. Locomotion was the biological process mainly associated to CMs-T and reproduction to CM-T3. Immune responses were enriched in CMs-T. Extracellular matrix and structural proteins were associated to CMs-T. CM-T3 was enriched in proteins with catalytic activity and CM-T2 in proteins with defense/immunity activity. Metabolism and energy pathways were enriched in CM-T3 and those with immune system functions in CMs-T. Transport proteins were enriched in CM-T2 and CM-BPH.
Proteins and pathways reported in this study could be useful to distinguish stages of disease and may become targets for novel therapies.
背景/目的：前列腺周围脂肪组织（PPAT）引导肿瘤行为。微环境分泌组提供了与其生物学相关的信息。本研究旨在鉴定来自前列腺癌和良性前列腺增生（BPH）患者的PPAT分泌蛋白。
对前列腺癌患者（CMs-T）（T3期：CM-T3，T2期：CM-T2）或良性疾病患者（CM-BPH）的PPAT条件培养基（CM）进行基于液相色谱-质谱的蛋白质组学分析。
在CM-T3中鉴定出的蛋白质数量最多且种类最多。运动是主要与CMs-T相关的生物学过程，而生殖与CM-T3相关。免疫反应在CMs-T中富集。细胞外基质和结构蛋白与CMs-T相关。CM-T3富含具有催化活性的蛋白质，而CM-T2富含具有防御/免疫活性的蛋白质。代谢和能量途径在CM-T3中富集，而免疫系统功能相关的途径在CMs-T中富集。转运蛋白在CM-T2和CM-BPH中富集。
本研究报道的蛋白质和途径可能有助于区分疾病阶段，并可能成为新疗法的靶点。
