自然杀伤（CD161）细胞参与Lewis大鼠肉瘤自发消退的免疫组化证据
Immunohistochemical Evidence of the Involvement of Natural Killer (CD161) Cells in Spontaneous Regression of Lewis Rat Sarcoma.
作者信息
Kovalska Jana, Cervinkova Monika, Chmelikova Eva, Planska Daniela, Cizkova Jana, Horak Vratislav
机构信息
Institute of Animal Physiology and Genetics, the Czech Academy of Sciences, Libechov, Czech Republic
Department of Veterinary Sciences, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, Prague, Czech Republic.
出版信息
In Vivo. 2019 Jan-Feb;33(1):47-52. doi: 10.21873/invivo.11437.
BACKGROUND/AIM: Spontaneous regression (SR) of tumours is a rare phenomenon not yet fully understood. The aim of this study was to investigate immune cells infiltrating progressive and SR tumours in a Lewis rat sarcoma model.
MATERIALS AND METHODS
Rats were subcutaneously inoculated with rat sarcoma R5-28 (clone C4) cells. Developing tumours were obtained on day 42 and cryosections were immunohistochemically processed for detection of immune cells.
RESULTS
A high density of granulocytes was found in the necrotic areas of both progressive and SR tumours. CD4 cells and CD8 cells were rare and sparsely dispersed in the tumour tissue without clear difference between the two types of tumours. On the contrary, CD161 cells were abundant and evenly distributed in SR tumours, but these cells were very rare in progressive tumours.
CONCLUSION
Based on the differences in number and distribution of the immune cell subpopulations, we believe that natural killer (CD161) cells play a major role in the destruction of cancer cells during SR of tumours in this Lewis rat model.
背景/目的：肿瘤的自发消退（SR）是一种尚未完全了解的罕见现象。本研究的目的是在Lewis大鼠肉瘤模型中研究浸润进展期和自发消退肿瘤的免疫细胞。
材料与方法
将大鼠肉瘤R5-28（克隆C4）细胞皮下接种于大鼠。在第42天获取正在生长的肿瘤，并对冰冻切片进行免疫组织化学处理以检测免疫细胞。
结果
在进展期和自发消退肿瘤的坏死区域均发现高密度的粒细胞。CD4细胞和CD8细胞稀少且稀疏地分散在肿瘤组织中，两种类型的肿瘤之间无明显差异。相反，CD161细胞在自发消退肿瘤中丰富且均匀分布，但在进展期肿瘤中非常罕见。
结论
基于免疫细胞亚群数量和分布的差异，我们认为在该Lewis大鼠模型中，自然杀伤（CD161）细胞在肿瘤自发消退过程中对癌细胞的破坏起主要作用。
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