Press O W, Rosse C
Immunology. 1978 Mar;34(3):539-47.
Radioautographic analyses were performed on PHA stimulated cultures of in vivo labelled cells obtained from mice previously injected with 3H-TdR to selectively label either cells with a rapid renewal rate (RR) or a slow renewal rate (SR). ha responsive cells in the bone marrow (BM) were found to be virtually all RR cells, whereas both RR and SR cells from lymph nodes (LN) and spleen (Spl) were stimulated by this mitogen. However, RR cells were proportionately more responsive to PHA than SR cells in all tissues examined. Only one out of 200 BM PHA blasts belonged to the SR subclass, whereas the RR/SR ratio was approximately 1/1 for LN and 2/1 for Spl. Control experiments demonstrated that significant in vitro re-utilization of 3H-TdR from dying cells did not occur in the cultures. These results support a growing body of evidence that BM PHA responsive cells are precursor T-cells which are known to have a rapid turnover rate.
对先前注射了3H-TdR的小鼠体内标记细胞的PHA刺激培养物进行放射自显影分析，以选择性标记快速更新率（RR）或缓慢更新率（SR）的细胞。发现骨髓（BM）中的ha反应性细胞几乎都是RR细胞，而来自淋巴结（LN）和脾脏（Spl）的RR和SR细胞均受该有丝分裂原刺激。然而，在所有检查的组织中，RR细胞对PHA的反应比SR细胞更敏感。200个BM PHA母细胞中只有1个属于SR亚类，而LN的RR/SR比率约为1/1，Spl的RR/SR比率为2/1。对照实验表明，培养物中未发生来自死亡细胞的3H-TdR的显著体外再利用。这些结果支持了越来越多的证据，即BM PHA反应性细胞是已知具有快速更新率的前体T细胞。
