Press O W, Rosse C, Clagett J
J Exp Med. 1977 Sep 1;146(3):735-46. doi: 10.1084/jem.146.3.735.
The cells in mouse bone marrow (BM) capable of responding to phytohemagglutinin (PHA) were shown to be precursor T cells in experiments employing athymic mice, immunofluorescence, and specific lysis of T or B cells with cytotoxic antisera + complement. In contrast, the responses of lymph node (LN) and spleen (Spl) cells to this mitogen were shown by the same techniques to rely upon resident populations of mature T lymphocytes in these peripheral lymphoid organs. Cytolysis of T cells with anti-theta (anti-Thy 1), anti-thymocyte, or anti-brain antisera abolished the PHA responses of LN and Spl, but had no appreciable effect on the BM PHA response. Lysis of B cells with anti-mouse gamma globulin or anti-mouse IgM antisera had no significant effect on either Spl or BM blastogenesis in response to this lectin. Immunofluorescent studies with fluoresceinated anti-brain sera demonstrated acquisition of T-cell surface antigens by BM null lymphocytes during the blastogenic response of this tissue to PHA. The results of these immunofluorescence experiments were reproducible even when marrow obtained from nude mice and pretreated with anti-brain serum plus complement was employed. The implications of these findings with regard to prophylaxis against graft versus host disease in BM transplant recipients are discussed.
在使用无胸腺小鼠、免疫荧光以及用细胞毒性抗血清加补体对T细胞或B细胞进行特异性裂解的实验中，已证明小鼠骨髓（BM）中能够对植物血凝素（PHA）作出反应的细胞是T细胞前体。相比之下，采用相同技术表明，淋巴结（LN）和脾（Spl）细胞对这种促有丝分裂原的反应依赖于这些外周淋巴器官中成熟T淋巴细胞的驻留群体。用抗θ（抗Thy 1）、抗胸腺细胞或抗脑抗血清对T细胞进行细胞裂解可消除LN和Spl的PHA反应，但对BM的PHA反应没有明显影响。用抗小鼠γ球蛋白或抗小鼠IgM抗血清对B细胞进行裂解对Spl或BM对该凝集素的增殖反应均无显著影响。用荧光素化抗脑血清进行的免疫荧光研究表明，在该组织对PHA的增殖反应过程中，BM空淋巴细胞获得了T细胞表面抗原。即使使用从裸鼠获得并用抗脑血清加补体预处理的骨髓，这些免疫荧光实验的结果也是可重复的。讨论了这些发现对预防骨髓移植受者移植物抗宿主病的意义。
