当心缺口。

Mind the nick.

机构信息

a Departamento de Genetica , Universidad de Sevilla , Sevilla , Spain.

b Centro Andaluz de Biología Molecular y Medicina Regenerativa-CABIMER , Universidad de Sevilla-CSIC-Universidad Pablo de Olavide , Sevilla , Spain.

出版信息

Cell Cycle. 2019 Jan;18(1):115-117. doi: 10.1080/15384101.2018.1558861. Epub 2018 Dec 28.

DOI:10.1080/15384101.2018.1558861

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6343732/

Abstract

Genomic DNA can be prone to endogenous nicks. The contribution of DNA nicks to genome instability includes the breakage of double-stranded DNA due to single-stranded DNA nicking. A recent study mapped the genome-wide distribution of endogenous DNA nicks suggesting that transcription contributes to the formation and distribution of DNA nicks. R-loops are a byproduct of transcription, and nicked DNA seems to contribute to R-loop formation and vice versa. Here, I want discuss the possibility that the 3'OH of nicked DNA at R-loops could trigger unscheduled replication events.

摘要

基因组 DNA 容易受到内源性缺口的影响。DNA 缺口对基因组不稳定性的贡献包括由于单链 DNA 缺口导致双链 DNA 的断裂。最近的一项研究绘制了全基因组范围内内源性 DNA 缺口的分布图谱，表明转录有助于 DNA 缺口的形成和分布。R 环是转录的副产物，而缺口 DNA 似乎有助于 R 环的形成，反之亦然。在这里，我想讨论 R 环中缺口 DNA 的 3'OH 是否可能引发无计划的复制事件。

相似文献

1

Mind the nick.当心缺口。

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2

Endogenous single-strand DNA breaks at RNA polymerase II promoters in Saccharomyces cerevisiae.酿酒酵母 RNA 聚合酶 II 启动子处的内源性单链 DNA 断裂。

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3

Breakage of double-stranded DNA due to single-stranded nicking.由于单链切口导致的双链DNA断裂。

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4

R-loops and nicks initiate DNA breakage and genome instability in non-growing Escherichia coli.R 环和缺口会引发非生长状态下大肠杆菌的 DNA 断裂和基因组不稳定性。

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Ddc1 checkpoint protein and DNA polymerase ɛ interact with nick-containing DNA repair intermediate in cell free extracts of Saccharomyces cerevisiae.在酿酒酵母无细胞提取物中，Ddc1 检查点蛋白和 DNA 聚合酶ɛ与含缺口的 DNA 修复中间体相互作用。

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8

Effects of nicks on repair of single-stranded loops in heteroduplex DNA in mammalian cells.切口对哺乳动物细胞异源双链DNA中单链环修复的影响。

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9

RNase H enables efficient repair of R-loop induced DNA damage.核糖核酸酶H能够有效修复R环诱导的DNA损伤。

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10

Studying repair of a single protein-bound nick in vivo using the Flp-nick system.利用Flp切口系统在体内研究单个蛋白质结合切口的修复。

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本文引用的文献

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Endogenous single-strand DNA breaks at RNA polymerase II promoters in Saccharomyces cerevisiae.酿酒酵母 RNA 聚合酶 II 启动子处的内源性单链 DNA 断裂。

Nucleic Acids Res. 2018 Nov 16;46(20):10649-10668. doi: 10.1093/nar/gky743.

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RNase H enables efficient repair of R-loop induced DNA damage.核糖核酸酶H能够有效修复R环诱导的DNA损伤。

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DNA break mapping reveals topoisomerase II activity genome-wide.DNA断裂图谱揭示了全基因组范围内的拓扑异构酶II活性。

Int J Mol Sci. 2014 Jul 23;15(7):13111-22. doi: 10.3390/ijms150713111.

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Single-strand interruptions in replicating chromosomes cause double-strand breaks.复制染色体中的单链中断会导致双链断裂。

Proc Natl Acad Sci U S A. 2001 Jul 17;98(15):8241-6. doi: 10.1073/pnas.131009198.

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