Biotherapy Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, China; Engineering Key Laboratory for Cell Therapy of Henan Province, Zhengzhou, Henan 450052, China.
Department of Gastroenterology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, China.
Cell Immunol. 2019 Feb;336:34-39. doi: 10.1016/j.cellimm.2018.12.007. Epub 2018 Dec 20.
T cell receptors (TCR) diversity is known to serve as a defining hallmark of the antigen-reactive T cell repertoire. Complementarity determining region 3 (CDR3) was the most important region for the recognition of peptide-major histocompatibility complex (MHC) complexes and represented the diversity of TCR repertoire. In this study, we detected the CDR3 spectratypes by complexity scoring system to assess TCR repertoire diversity and further analyzed the correlation of CDR3 score with CD8+ T cell function and with the prognosis of chronic hepatitis C virus (HCV)-infected patients. The results demonstrated that CDR3 score was related to CD8+ T cell function and prognosis by analyzing the clinical indicators such as viral load (VL), rapid virologic response (RVR), early virologic response (EVR) and sustained virologic response (SVR). Importantly, we found that Vβ27, a member of CDR3 subfamily, might play an important role in the clearance of HCV. These findings indicate that TCR diversity maybe serve as a biomarker to predict the clinical parameters of HCV-infected patients.
T 细胞受体 (TCR) 的多样性被认为是抗原反应性 T 细胞库的一个重要特征。互补决定区 3 (CDR3) 是识别肽-主要组织相容性复合物 (MHC) 复合物的最重要区域，代表了 TCR 库的多样性。在这项研究中，我们通过复杂度评分系统检测 CDR3 谱型，以评估 TCR 库的多样性，并进一步分析 CDR3 评分与 CD8+ T 细胞功能以及慢性丙型肝炎病毒 (HCV) 感染患者预后的相关性。结果表明，通过分析病毒载量 (VL)、快速病毒学应答 (RVR)、早期病毒学应答 (EVR) 和持续病毒学应答 (SVR) 等临床指标，CDR3 评分与 CD8+ T 细胞功能和预后相关。重要的是，我们发现 CDR3 亚家族的成员 Vβ27 可能在 HCV 的清除中发挥重要作用。这些发现表明 TCR 多样性可能作为预测 HCV 感染患者临床参数的生物标志物。
