Zahra Andleeb, Hussain Bilal, Jamil Amer, Ahmed Z, Mahboob Shahid
Department of Bioinformatics, Government College, University Faisalabad, Pakistan.
Department of Zoology, Government College University, Faisalabad, Pakistan.
Saudi J Biol Sci. 2018 Dec;25(8):1720-1723. doi: 10.1016/j.sjbs.2018.10.001. Epub 2018 Oct 2.
In forensic science, the human identification is the major goal in criminal cases and in paternity dispute, based on DNA profiling of individual. Analysis of short tandem repeat (STRs) markers used as a reliable technique for this purpose. In this study the main objective was to develop a human identification based on DNA fingerprinting using Human Identification Barcode System (HIBS). HIBS may be used to provide a unique molecular signature of human in the form of a barcode. DNA was isolated from blood by using PCR technique to detect bands to design human barcode using self-designed system. HIBS is a web based application that can be accessed via www.hibs.com.pk. HIBS can be accessed with internet access and may be introduced on security checkpoints to identify an individual based on his own DNA instead of conventional procedures of identification. The barcode generated through DNA fingerprinting will be stored in a HIBS, and may also be a part of CNIC. It may be successfully used against suicide bombers, target killers, etc., as even a single blood spot, a few skin cells, root of the hair etc., to identify such culprits. It may also be effectively used to relieve the individuals with false accusations.
在法医学中,基于个体的DNA分析,人类身份鉴定是刑事案件和亲子关系纠纷中的主要目标。短串联重复序列(STRs)标记分析是用于此目的的可靠技术。本研究的主要目的是利用人类身份识别条形码系统(HIBS)开发基于DNA指纹识别的人类身份鉴定方法。HIBS可用于以条形码的形式提供人类独特的分子特征。通过PCR技术从血液中分离DNA,以检测条带,使用自行设计的系统设计人类条形码。HIBS是一个基于网络的应用程序,可通过www.hibs.com.pk访问。通过互联网接入即可访问HIBS,并且可以在安全检查点引入该系统,以便基于个体自身的DNA而非传统的身份识别程序来识别个体。通过DNA指纹识别生成的条形码将存储在HIBS中,也可能成为计算机化国民身份卡(CNIC)的一部分。它可以成功地用于对付自杀式炸弹袭击者、目标杀手等,因为即使是单个血斑、一些皮肤细胞、发根等,也能识别此类罪犯。它还可以有效地用于为被错误指控的个人洗脱罪名。