Aksenov I V, Avren'eva L I, Guseva G V, Trusov N V, Balakina A S, Mzhel'skaya K V, Soto C J, Kravchenko L V, Tutelyan V A
Federal Research Centre of Nutrition, Biotechnology and Food Safety, Moscow.
I.M. Sechenov First Moscow State Medical University.
Vopr Pitan. 2018;87(5):6-12. doi: 10.24411/0042-8833-2018-10047. Epub 2018 Sep 13.
The purpose of the study was to determine effects of quercetin on protective capacity parameters in the experiment on rats fed a high fructose diet. Rats of the control group received a semi-synthetic (s/s) diet and water; animals from the 1st experimental group - s/s diet and 20% fructose solution instead of drinking water; rats of the 2nd experimental group- s/s diet with quercetin (0.1% indiet) and 20% fructose solution instead of drinking water for 20 weeks. Parameters of antioxidant status [total antioxidant activity (AOA), the content of malondialdehyde (MDA) and lipids hydroperoxides, the level of reduced and oxidized glutathione, activity of superoxide dismutase, catalase, glutathione peroxidase, paraoxonase-1, hemeoxygenase-1, NAD(P)H-quinone oxidoreductase], the activity of xenobiotic-metabolizing enzymes [CYP1A1, CYP1A2, CYP2B1, CYP3A, UDP-glucuronosyltransferase (UDP-GT) and glutathione transferase] were studied in plasma and liver of rats. Consumption of the high-fructose diet led to changes in some parameters: diminution of AOA in blood plasma, decrease of AOA and MDA level, unsedimentable activity of lysosomal enzymes, increase of the UDP-GT activity in liver. The inclusion of quercetin in the diet did not affect the studied parameters, except for a more pronounced decrease of the unsedimentable activity of lysosomal enzymes in rat liver. The results of the study indicated that there was no significant effect of quercetin on the protective capacity of rats at the initial stage of obesity caused by high-fructose diet.
本研究的目的是确定槲皮素对喂食高果糖饮食的大鼠实验中保护能力参数的影响。对照组大鼠接受半合成（s/s）饮食和水；第一实验组的动物——s/s饮食，用20%果糖溶液代替饮用水；第二实验组的大鼠——s/s饮食加槲皮素（饮食中含0.1%），用20%果糖溶液代替饮用水，持续20周。研究了大鼠血浆和肝脏中抗氧化状态参数[总抗氧化活性（AOA）、丙二醛（MDA）和脂质氢过氧化物含量、还原型和氧化型谷胱甘肽水平、超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、对氧磷酶-1、血红素加氧酶-1、NAD（P）H-醌氧化还原酶活性]、外源性代谢酶[细胞色素P450 1A1（CYP1A1）、细胞色素P450 1A2（CYP1A2）、细胞色素P450 2B1（CYP2B1）、细胞色素P450 3A（CYP3A）、尿苷二磷酸葡萄糖醛酸基转移酶（UDP-GT）和谷胱甘肽转移酶]的活性。食用高果糖饮食导致一些参数发生变化：血浆中AOA降低、AOA和MDA水平下降、溶酶体酶的不可沉淀活性降低、肝脏中UDP-GT活性增加。饮食中添加槲皮素除了使大鼠肝脏中溶酶体酶的不可沉淀活性更显著降低外，对所研究的参数没有影响。研究结果表明，在高果糖饮食导致肥胖的初始阶段，槲皮素对大鼠的保护能力没有显著影响。
