Aksenov I V, Trusov N V, Avren'eva L I, Guseva G V, Lashneva N V, Kravchenko L V, Tutel'ian V A
Vopr Pitan. 2014;83(5):4-11.
The study has been carried out on 6 groups of male Wistar rats, which received semi-synthetic diets within 28 days. Rats of 1st and 4th group received fat-free diet, 2nid.and 5th - diet containing standard amount of fat (10% by weight, 26% by caloric content; lard/sunflower oil - 1/1); 3rd and 6th group - a high-fat diet (30% by weight, 56% by caloric content). During the last 14 days of the experiment rats received rutin in the dose of 40 mg/kg b.w. AOA, MDA level and the activity of paraoxonase I have been evaluated in blood serum. In rat liver along with the parameters of the antioxidant status (MDA level, activity of paraoxonase 1, quinone reductase, heme oxygenase-1) the activity of xenobiotic-metabolizing enzymes (XME) (CYP1A1, CYP1A2, CYP3A1, CYP2B1, UDP-glucuronosyl transferase and glutathione transferase) and the activity of lysosomal enzymes (arylsulfatase A and B, β-galactosidase and β-glucuronidase) have been investigated. Elevation of the activity of antioxidant enzymes and XME in liver with the increase of diet fat content has been-noted. Rutin admihistration had no effect onparamete6rs of antioxidant status and decreased unsedimentable activity of lysosomal enzymes that did not depend on fat content in the diet. Rutin receiving increased the activity of all studied XME in rats fed standard diet, but practically did not effect on their activity in rats fed by fat-free and high-fat diets. Thus, rutin in pharmacological dose has no effect on the activity of antioxidant enzymes that doesn't depend on the level of fat in the diet, while the decrease or increase of diet fat content modulates (weakens) the influence of rutin on the XME activity.
该研究对6组雄性Wistar大鼠进行，这些大鼠在28天内接受半合成饮食。第1组和第4组大鼠接受无脂饮食，第2组和第5组大鼠接受含标准量脂肪的饮食（重量比10%，热量比26%；猪油/葵花籽油 - 1/1）；第3组和第6组大鼠接受高脂饮食（重量比30%，热量比56%）。在实验的最后14天，大鼠接受剂量为40mg/kg体重的芦丁。评估了血清中的AOA、MDA水平和对氧磷酶I的活性。在大鼠肝脏中，除了抗氧化状态参数（MDA水平、对氧磷酶1、醌还原酶、血红素加氧酶-1的活性）外，还研究了外源性物质代谢酶（XME）（CYP1A1、CYP1A2、CYP3A1、CYP2B1、UDP-葡萄糖醛酸转移酶和谷胱甘肽转移酶）的活性以及溶酶体酶（芳基硫酸酯酶A和B、β-半乳糖苷酶和β-葡萄糖醛酸酶）的活性。随着饮食脂肪含量的增加，肝脏中抗氧化酶和XME的活性升高。芦丁给药对抗氧化状态参数没有影响，并降低了不依赖于饮食脂肪含量的溶酶体酶的不可沉降活性。接受芦丁增加了喂食标准饮食大鼠中所有研究的XME的活性，但实际上对喂食无脂和高脂饮食大鼠的活性没有影响。因此，药理剂量的芦丁对不依赖于饮食脂肪水平的抗氧化酶活性没有影响，而饮食脂肪含量的降低或增加调节（减弱）了芦丁对XME活性的影响。
