Liu Lin-Lin, Li Hui, He Dan, Wang Meng-Lei, Liu Zhen-Li, Shu Yi-Song, Zhao Si-Yu, Liu Yuan-Yan
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China.
Institution of Basic Theory, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Zhongguo Zhong Yao Za Zhi. 2018 Nov;43(22):4519-4527. doi: 10.19540/j.cnki.cjcmm.20180815.001.
This present study was to investigate the metabolism and excretion of characteristic polyphenols such as flavonoids and coumarins in urine and feces of rats after intragastric administration of ethanol extracts of Citri Reticulatae Pericarpium Viride and Citri Reticulatae Pericarpium. The urine and feces of rats were collected after intragastric administration of 70% ethanol extracts of Citri Reticulatae Pericarpium Viride and Citri Reticulatae Pericarpium. Rapid resolution liquid chromatography coupled with quadrupole tandem mass spectrometry (RRLC-QqQ-MSn) was applied to compare the contents of polyphenols in ethanol extract, urine and feces. By comparing with reference substance, 30 polyphenols were identified from the ethanol extracts of Citri Reticulatae Pericarpium Viride and Citri Reticulatae Pericarpium, including flavone glycosides, flavones, flavonone glycosides, flavonones, flavonol glycosides, polymethoxyflavones, coumarins, and limonoids and so on. The detection of various types of compounds showed differences in contents between the intestinal metabolism and excretion in the feces after systemic circulatory metabolism and renal excretion. The results showed that the polymethoxyflavones and flavonones were primarily excreted through urine, and the flavonone glycosides and limonoids were primarily excreted through feces. However, coumarins were hardly detected in feces and urine, indicating that coumarins may be metabolized in the body.
本研究旨在探讨大鼠灌胃青皮和陈皮乙醇提取物后，尿液和粪便中黄酮类、香豆素类等特征性多酚的代谢及排泄情况。灌胃青皮和陈皮70%乙醇提取物后，收集大鼠尿液和粪便。采用快速液相色谱-四极杆串联质谱联用技术（RRLC-QqQ-MSn）比较乙醇提取物、尿液和粪便中多酚的含量。通过与对照品比较，从青皮和陈皮乙醇提取物中鉴定出30种多酚，包括黄酮苷、黄酮、黄酮醇苷、黄酮醇、多甲氧基黄酮、香豆素和柠檬苦素等。各类化合物的检测结果表明，经全身循环代谢和肾脏排泄后，粪便中的肠道代谢和排泄在含量上存在差异。结果显示，多甲氧基黄酮和黄酮醇主要通过尿液排泄，黄酮醇苷和柠檬苦素主要通过粪便排泄。然而，在粪便和尿液中几乎未检测到香豆素，表明香豆素可能在体内发生了代谢。
