[Effects of different aerobic exercise time on myocardial cell apoptosis in mice].

OBJECTIVE

To compare the effects of different aerobic exercise time on cardiomyocyte apoptosis and its mechanism in rats.

METHODS

80 male clean grade BALB/c mice were split into short-period quiet group, long-period quiet group, shortperiod exercise group and long-period exercise group, each of 20 mice. The feeding and aerobic training time of mice in short-term quiet group and short-term exercise group was 3 months, and the time of feeding and aerobic training in long-term quiet group and longterm exercise group was 6 months. The TUNE kit was used to detect the apoptosis of cardiac myocytes, and the mRNA and protein expression of Bcl-2 and Bax mRNA in cardiac myocytes were assessed by RT-PCR and Western blot method.

RESULTS

The cardiomyocyte apoptosis index of mice in the long-period exercise group was significantly lower than those in the short-period aerobic training mice and the long-period and shortperiod quiet group. The levels of Bcl-2 mRNA and protein expression in their myocardium were significantly higher than those in the long-period and short-period quiet group( P <0. 05), and the Bax mRNA and protein expression were significantly lower than those inthe long-period and short-period quiet group( P < 0. 05). The Bcl-2/Bax mRNA mRNA and protein ratio of the long-term exercise group were significantly improved compared with the short-term group( P < 0. 05). The myocardial apoptosis index and the expression of Bcl-2 and Bax in the short-period exercise group were improved compared with those in the short-period quiet group, but the difference was not obvious.

CONCLUSION

6 months of aerobic exercise can effectively prevent cardiomyocyte apoptosis in mice. It is an important way to effectively regulate Bcl-2, Bax expression and Bcl-2/Bax level of cardiomyocytes.