Yue Qianlan, Pan Xiangxiang, Liu Yunjie, Chen Tao, Zhang Lishi, Wang Rui
Sichuan Center for Disease Control and Prevention, Chengdu 610041, China.
Wei Sheng Yan Jiu. 2018 Sep;47(5):798-803.
To study the protective effects of two deep sea water( DSW)samples with different ion concentration against sub-acute alcoholic liver injury.
A total of 72 male KM mice( 18-22 g) were randomly assigned into six groups, i. e. the control group, model group, DSW-A1 fold concentration group( A1), DSW-A5 folds concentration group( A5), DSW-A 10 folds concentration group( A10), DSW-B5 fold concentration group( B). The corresponding DSW solution was ingested freely by each DSW group, and deionized water was ingested freely by the other two groups. The total administration duration was 30 consecutive days. Groups were given 50% ethanol solution( 0. 10 m L/10( g·d)) by ig since the 15 th day except the control group. After administration period, detect serum aspartate aminotransferase( AST), alanine aminotransferase( ALT), total bilirubin( T-BIL), total cholesterol( TC), triglyceride( TG) and low density lipoprotein( LDL), liver TC, TG, GSH, malonaldehyde( MDA), glutathione/glutathione oxidized( GSH/GSSG), superoxide dismutase( SOD), glutathione peroxidase( GSH-Px), catalase( CAT), xanthine oxidase( XOD). Livers were also further detected by microscopic examinations.
Compared with the control group, the model group had serious liver steatosis. The histological score and serum T-BIL were significantly increased( P < 0. 01), serum TC, LDL, AST and liver MDA were significantly increased( P < 0. 05), liver GSH-Px was significantly decreased( P < 0. 01). Compared with the model group, in A1 group, the serum T-BIL was significantly decreased( P < 0. 05), liver GSH-Px( P < 0. 01) and XOD( P < 0. 05) were significantly increased. In A5 group, serum T-BIL, liver MDA and pathologic changes were significantly decreased( P < 0. 01), serum TG and TC were significantly decreased( P <0. 05), liver GSH( P < 0. 01), GSH/GSSG( P < 0. 05), GSH-Px( P < 0. 01) and XOD( P < 0. 01) were significantly increased. In A10 group, serum LDL and AST were significantly decreased( P < 0. 05), serum TG, TC, T-BIL, liver MDA and the pathologic changes were significantly decreased( P < 0. 01), liver GSH/GSSG( P < 0. 05) and GSHPx( P < 0. 01) were significantly increased. In B group, serum TC and liver MDA were significantly decreased( P < 0. 05), serum TG, T-BIL, AST and liver pathologic changes were significantly decreased( P < 0. 01), liver XOD( P < 0. 05) and GSH-Px( P< 0. 01) were significantly increased.
Middle and high concentration DSWA and DSW-B have protective effects against sub-acute alcoholic liver injury by decreasing serum lipid level, and improving the ability of antioxidation.
研究两种离子浓度不同的深海海水（DSW）样品对亚急性酒精性肝损伤的保护作用。
将72只雄性KM小鼠（18 - 22 g）随机分为6组，即对照组、模型组、DSW - A1倍浓度组（A1）、DSW - A5倍浓度组（A5）、DSW - A10倍浓度组（A10）、DSW - B5倍浓度组（B）。各DSW组小鼠自由饮用相应的DSW溶液，另外两组自由饮用去离子水。给药总时长为连续30天。除对照组外，自第15天起各实验组小鼠每天经口灌胃给予50%乙醇溶液（0.10 mL/10(g·d)）。给药期结束后，检测血清天冬氨酸氨基转移酶（AST）、丙氨酸氨基转移酶（ALT）、总胆红素（T - BIL）、总胆固醇（TC）、甘油三酯（TG）和低密度脂蛋白（LDL），肝脏TC、TG、谷胱甘肽（GSH）、丙二醛（MDA）、还原型谷胱甘肽/氧化型谷胱甘肽（GSH/GSSG）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH - Px）、过氧化氢酶（CAT）、黄嘌呤氧化酶（XOD）。同时对肝脏进行进一步的显微镜检查。
与对照组相比，模型组肝脏出现严重脂肪变性。组织学评分和血清T - BIL显著升高（P < 0.01），血清TC、LDL、AST和肝脏MDA显著升高（P < 0.05），肝脏GSH - Px显著降低（P < 0.01）。与模型组相比，A1组血清T - BIL显著降低（P < 0.05），肝脏GSH - Px（P < 0.01）和XOD（P < 0.05）显著升高。A5组血清T - BIL、肝脏MDA和病理变化显著降低（P < 0.01），血清TG和TC显著降低（P < 0.05），肝脏GSH（P < 0.01）、GSH/GSSG（P < 0.05）、GSH - Px（P < 0.01）和XOD（P < 0.01）显著升高。A10组血清LDL和AST显著降低（P < 0.05），血清TG、TC、T - BIL、肝脏MDA和病理变化显著降低（P < 0.01），肝脏GSH/GSSG（P < 0.05）和GSH - Px（P < 0.01）显著升高。B组血清TC和肝脏MDA显著降低（P < 0.05），血清TG、T - BIL、AST和肝脏病理变化显著降低（P < 0.01），肝脏XOD（P < 0.05）和GSH - Px（P < 0.01）显著升高。
中高浓度的DSW - A和DSW - B通过降低血脂水平和提高抗氧化能力对亚急性酒精性肝损伤具有保护作用。
Zotero 插件