Key Laboratory of Ministry of Education for Medicinal Resource and Natural Pharmaceutical Chemistry, College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an 710062, China.
Food Funct. 2015 Dec;6(12):3760-9. doi: 10.1039/c5fo00612k. Epub 2015 Sep 28.
This study was designed to investigate the protective effects of d-Chiro-Inositol (DCI) enriched tartary buckwheat extract (DTBE) against high fructose (HF) diet-induced hyperglycemia and liver injury in mice. HPLC analysis revealed that the content of DCI present in purified DTBE was 34.06%. Mice fed 20% fructose in drinking water for 8 weeks significantly displayed hyperglycemia, hyperinsulinemia, dyslipidemia, hepatic steatosis and oxidative stress (p < 0.01). Continuous administration of DTBE in HF-fed mice dose-dependently reduced the HF-induced elevation of body weight, serum glucose, insulin, total cholesterol (TC), total triglycerides (TG) and low density lipoprotein cholesterol (LDL-C) levels, as well as serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), C-reactive protein (CRP) and lactate dehydrogenase (LDH) activities, while the HF-induced decline of serum high density lipoprotein-cholesterol (HDL-C) levels could be markedly elevated in the mice. Meanwhile, DTBE also dose-dependently increased the hepatic total superoxide dismutase (T-SOD) and glutathione peroxidase (GSH-Px) activities, and decreased hepatic malonaldehyde (MDA) levels, relative to HF-treated mice. Histopathology of H&E and Oil Red O staining confirmed liver injury induced by a HF diet and the hepatoprotective effect of DTBE. These findings are the first to demonstrate that the intake of DTBE may be a feasible preventive or therapeutic strategy for HF diet-induced hyperglycemia, hepatic steatosis and oxidative injury.
本研究旨在探讨富含 D-手性肌醇（DCI）的苦荞提取物（DTBE）对高果糖（HF）饮食诱导的小鼠高血糖和肝损伤的保护作用。HPLC 分析显示，纯化 DTBE 中 DCI 的含量为 34.06%。用含 20%果糖的饮用水喂养 8 周的小鼠显著表现出高血糖、高胰岛素血症、血脂异常、肝脂肪变性和氧化应激（p<0.01）。连续给予 DTBE 可剂量依赖性地降低 HF 喂养小鼠的 HF 诱导的体重、血清葡萄糖、胰岛素、总胆固醇（TC）、总甘油三酯（TG）和低密度脂蛋白胆固醇（LDL-C）水平，以及血清丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、C-反应蛋白（CRP）和乳酸脱氢酶（LDH）活性，同时可显著升高 HF 诱导的血清高密度脂蛋白胆固醇（HDL-C）水平下降。同时，DTBE 还可剂量依赖性地增加肝总超氧化物歧化酶（T-SOD）和谷胱甘肽过氧化物酶（GSH-Px）活性，降低肝丙二醛（MDA）水平，相对于 HF 治疗组小鼠。H&E 和油红 O 染色的组织病理学证实了 HF 饮食诱导的肝损伤和 DTBE 的保肝作用。这些发现首次表明，摄入 DTBE 可能是预防或治疗 HF 饮食诱导的高血糖、肝脂肪变性和氧化损伤的可行策略。
