Ligler F S, Cambier J C, Vitetta E S, Kettman J R, Uhr J W
J Immunol. 1978 Apr;120(4):1139-42.
The present study examines the isotype of cell surface immunoglobulins involved in triggering a response to thymus-dependent and thymus-independent antigens. Antibodies to immunoglobulin isotypes were used to block the in vitro interaction of receptor IgD and IgM with antigen. The results indicate that both IgD and IgM are necessary to trigger a response to TNP-SRBC but that only IgM is required for responsiveness to TNP-Brucella. Limiting dilution studies indicate that the inhibition of the immune response by antibody occurs at the level of precursor activation and suggest that there is no effect of antibody on subsequent proliferation of the daughter cells.
本研究检测了参与引发对胸腺依赖性和胸腺非依赖性抗原反应的细胞表面免疫球蛋白的同种型。针对免疫球蛋白同种型的抗体被用于阻断受体IgD和IgM与抗原的体外相互作用。结果表明，IgD和IgM对于引发对TNP-SRBC的反应都是必需的，但对TNP-布鲁氏菌的反应仅需要IgM。有限稀释研究表明，抗体对免疫反应的抑制发生在前体激活水平，并提示抗体对子代细胞随后的增殖没有影响。
