Mihara Yuki, Horikawa Makoto, Sato Shumpei, Eto Fumihiro, Hanada Mitsuru, Banno Tomohiro, Arima Hideyuki, Ushirozako Hiroki, Yamada Tomohiro, Xu Dongmin, Okamoto Ayako, Yamazaki Fumiyoshi, Takei Shiro, Omura Takao, Yao Ikuko, Matsuyama Yukihiro, Setou Mitsutoshi
Department of Orthopedic Surgery, Hamamatsu University School of Medicine, 1-20-1, Handayama, Higashi-ku, Hamamatsu, Shizuoka, 431-3192, Japan.
International Mass Imaging Center and Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka, 431-3192, Japan.
Neurosci Lett. 2019 Apr 17;698:69-75. doi: 10.1016/j.neulet.2018.12.035. Epub 2018 Dec 26.
In the current study, we aimed to analyze the lipid changes in the dorsal root ganglion (DRG) after sciatic nerve transection (SNT) using matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS). We found that the arachidonic acid-containing phosphatidylcholine (AA-PC), PC(16:0/20:4) largely increased, while PC(16:0/18:1), PC(18:0/18:1) and phosphatidic acid (PA)(36:2) levels largely decreased in the DRG following nerve injury. Previous studies show that the increase in PC(16:0/20:4) was associated with neuropathic pain and that decrease in PC(16:0/18:1), PC(18:0/18:1), and PA(36:2) were due to producing lysophosphatidic acid (LPA), an initiator for neuropathic pain. These results suggest that the lipid changes in DRG after SNT could be the result of changes for the cause of neuropathic pain. Thus, blocking of LPA could be potential for treatment of neuropathic pain.
在本研究中，我们旨在使用基质辅助激光解吸/电离成像质谱（MALDI-IMS）分析坐骨神经横断（SNT）后背根神经节（DRG）中的脂质变化。我们发现，含花生四烯酸的磷脂酰胆碱（AA-PC），即PC(16:0/20:4)大幅增加，而在神经损伤后，DRG中的PC(16:0/18:1)、PC(18:0/18:1)和磷脂酸（PA）(36:2)水平大幅下降。先前的研究表明，PC(16:0/20:4)的增加与神经性疼痛有关，而PC(16:0/18:1)、PC(18:0/18:1)和PA(36:2)的减少是由于产生了溶血磷脂酸（LPA），一种神经性疼痛的引发剂。这些结果表明，SNT后DRG中的脂质变化可能是神经性疼痛病因变化的结果。因此，阻断LPA可能具有治疗神经性疼痛的潜力。
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