无细胞微分隔转录-翻译用于合成通信网络的原型设计。
Cell-free microcompartmentalised transcription-translation for the prototyping of synthetic communication networks.
机构信息
Laboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of Technology, 5600 MB Eindhoven, The Netherlands; Institute for Complex Molecular Systems, Department of Biomedical Engineering, Eindhoven University of Technology, 5600 MB Eindhoven, The Netherlands; Computational Biology Group, Department of Biomedical Engineering, Eindhoven University of Technology, 5600 MB Eindhoven, The Netherlands.
Department of Biomedical Engineering & Department of Chemical Engineering and Chemistry, Eindhoven University of Technology, P.O. Box 513, 5600 MB Eindhoven, The Netherlands.
出版信息
Curr Opin Biotechnol. 2019 Aug;58:72-80. doi: 10.1016/j.copbio.2018.10.006. Epub 2018 Dec 26.
Recent efforts in synthetic biology have shown the possibility of engineering distributed functions in populations of living cells, which requires the development of highly orthogonal, genetically encoded communication pathways. Cell-free transcription-translation (TXTL) reactions encapsulated in microcompartments enable prototyping of molecular communication channels and their integration into engineered genetic circuits by mimicking critical cell features, such as gene expression, cell size, and cell individuality within a community. In this review, we discuss the uses of cell-free transcription-translation reactions for the development of synthetic genetic circuits, with a special focus on the use of microcompartments supporting this reaction. We highlight several studies where molecular communication between non-living microcompartments and living cells have been successfully engineered.
最近在合成生物学方面的努力表明，在活细胞群体中进行分布式功能工程的可能性，这需要开发高度正交的、遗传编码的通信途径。微隔室中封装的无细胞转录-翻译（TXTL）反应通过模拟关键的细胞特征，如基因表达、细胞大小和社区内的细胞个体性，使分子通信通道的原型设计和将其集成到工程遗传电路中成为可能。在这篇综述中，我们讨论了无细胞转录-翻译反应在合成遗传电路开发中的应用，特别关注支持这种反应的微隔室的使用。我们强调了几项研究，其中成功地设计了非活微隔室与活细胞之间的分子通信。
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