Saglie F R
UCLA School of Dentistry, Section of Periodontics 90024.
Scanning Microsc. 1988 Sep;2(3):1535-40.
During the past two decades there has been an increased understanding of bacterial invasion as a pathogenic mechanism of periodontal diseases. Scanning Electron Microscope (SEM) has played a key role in supporting the idea that bacterial invasion may be another pathogenic mechanism in periodontal disease. This has been due to the fact that SEM has a larger depth of focus and better resolving power than the Light Microscope (LM) and also allows for observation of rather large areas of tissue showing in depth the surface of the sample. This review deals with information obtained by using SEM as the fundamental method in studying and specifically identifying microorganisms within gingival tissues. New methodology using correlative microscopy for rapidly identifying invasive bacteria of periodontal tissues is discussed.
在过去二十年中，人们对细菌入侵作为牙周病的致病机制有了更深入的了解。扫描电子显微镜（SEM）在支持细菌入侵可能是牙周病的另一种致病机制这一观点方面发挥了关键作用。这是因为SEM比光学显微镜（LM）具有更大的焦深和更好的分辨能力，还能够观察相当大的组织区域，深入显示样本的表面。这篇综述涉及通过使用SEM作为研究和具体鉴定牙龈组织内微生物的基本方法所获得的信息。还讨论了使用相关显微镜快速鉴定牙周组织侵袭性细菌的新方法。
