好氧反硝化系统中添加氧化还原活性生物炭高效去除硝酸盐：分子和电化学机制。
Highly efficient nitrate removal in a heterotrophic denitrification system amended with redox-active biochar: A molecular and electrochemical mechanism.
机构信息
Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, 174 Shazheng Street, Chongqing 400044, China.
Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, 174 Shazheng Street, Chongqing 400044, China.
出版信息
Bioresour Technol. 2019 Mar;275:297-306. doi: 10.1016/j.biortech.2018.12.058. Epub 2018 Dec 17.
Biochar is widely used in water treatment because of its porous structure, however, the effects of biochars on denitrification remain unclear. Here, we combined molecular biological and electrochemical techniques to investigate effects of biochars (formed at 300 °C, 500 °C and 800 °C) on denitrification. Results showed that biochar at 300 °C increased total nitrogen removal by 415% and decreased NO accumulation by 78%. Mechanistic research demonstrated that it achieved the highest electron transfer efficiency and denitrifying enzyme activities. Further study evidenced that biochar at 300 °C increased the abundance of denitrifiers such as Pseudomonas. Correlation analysis indicated that nitrate reductase and nitrite reductase activities were the key factors influenced by biochar during denitrification. Overall, this study suggested that biochar at 300 °C could act as the bio-engineer of electron shuttle and the stimulator of denitrification, achieving high rate nitrogen removal and significant reduction of NO accumulation from high-strength wastewater.
生物炭由于其多孔结构而被广泛应用于水处理中，但生物炭对反硝化的影响尚不清楚。在这里，我们结合分子生物学和电化学技术研究了生物炭（在 300°C、500°C 和 800°C 下形成）对反硝化的影响。结果表明，300°C 的生物炭将总氮去除率提高了 415%，并将 NO 积累减少了 78%。机理研究表明，它实现了最高的电子传递效率和反硝化酶活性。进一步的研究表明，300°C 的生物炭增加了 Pseudomonas 等反硝化菌的丰度。相关性分析表明，硝酸盐还原酶和亚硝酸盐还原酶活性是反硝化过程中受生物炭影响的关键因素。总的来说，本研究表明，300°C 的生物炭可以作为电子穿梭体的生物工程菌和反硝化的刺激剂，实现从高强度废水中高效去除氮并显著减少 NO 积累。
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