McAteer J A, Dougherty G S, Gardner K D, Evan A P
Department of Anatomy, Indiana University School of Medicine, Indianapolis 46223.
Scanning Microsc. 1988 Sep;2(3):1739-63.
The purpose of this paper is to review in vitro cell and explant culture systems that exhibit epithelial cyst formation and that are used as models of polarized epithelial function. We examine a number of culture systems derived from a variety of cell and organ types, briefly describe the methodology and conditions used to establish these cultures and discuss aspects of the experimental application of each system. We conclude that the characteristics of epithelial cyst-forming cultures are dependent upon the origin and identity of the cell population, as well as the multiple factors that define the culture environment. Culture systems in which epithelial cyst development occurs provide tools to study fundamental problems in epithelial biology, such as the establishment and maintenance of cell polarity, cell recognition and cell sorting, also cell-specific functions involving solute and water transport and the production and modification of secreted products. In addition, epithelial cyst culture systems offer useful models to better understand cellular behavior in various pathologic conditions of cyst formation in man.
本文的目的是综述能够形成上皮囊肿且用作极化上皮功能模型的体外细胞和外植体培养系统。我们研究了多种源自不同细胞和器官类型的培养系统，简要描述了建立这些培养物所使用的方法和条件，并讨论了每个系统的实验应用方面。我们得出结论，上皮囊肿形成培养物的特性取决于细胞群体的来源和特性，以及定义培养环境的多种因素。发生上皮囊肿发育的培养系统为研究上皮生物学的基本问题提供了工具，例如细胞极性的建立和维持、细胞识别和细胞分选，以及涉及溶质和水转运以及分泌产物的产生和修饰的细胞特异性功能。此外，上皮囊肿培养系统提供了有用的模型，以更好地理解人类囊肿形成的各种病理状况下的细胞行为。
