Liming Shi, Guixia Li, Wenxin Shi, Guirong Tian
a Department of Clinical Laboratory , Heze municipal Hospital , Heze , China.
b Department of Scientific Research and Teaching , Heze municipal Hospital , Heze , China.
Exp Lung Res. 2018 Aug;44(6):263-271. doi: 10.1080/01902148.2018.1505976. Epub 2018 Dec 29.
Pulmonary infection with Klebsiella pneumoniae (KP) and carbapenem-resistant Klebsiella pneumoniae (CRKP) significantly contribute to morbidity and mortality in pneumonia. Recent studies have indicated that High-Mobility Group Box 1 Protein (HMGB1) plays an important role in the prevention and treatment of pneumonia. However the role of HMGB1 in CRKP-induced pneumonia has not been addressed. Materials andMethods: In vivo, we successfully established the KP and CRKP-induced pneumonia mouse model. We then tested the anti-HMGB1 IgG prevents CRKP-induced pneumonia.
The mice treated with the anti-HMGB1 IgG ameliorated CRKP-induced pulmonary infiltration of inflammatory cells, dissemination of bacteria and the cytokine storm by suppressing the HMGB1 signaling pathways.
These results indicate that HMGB1 may be an important contributor in these changes of CRKP-induced pneumonia. Thus, HMGB1 may provide a therapeutic target for reducing bacterial infection and lung inflammation in CRKP pneumonia.
肺炎克雷伯菌（KP）和耐碳青霉烯类肺炎克雷伯菌（CRKP）引起的肺部感染是导致肺炎发病和死亡的重要因素。最近的研究表明，高迁移率族蛋白B1（HMGB1）在肺炎的防治中发挥着重要作用。然而，HMGB1在CRKP所致肺炎中的作用尚未得到研究。材料与方法：在体内，我们成功建立了KP和CRKP诱导的肺炎小鼠模型。然后，我们测试了抗HMGB1 IgG对CRKP诱导的肺炎的预防作用。
用抗HMGB1 IgG治疗的小鼠通过抑制HMGB1信号通路，改善了CRKP诱导的肺部炎症细胞浸润、细菌播散和细胞因子风暴。
这些结果表明，HMGB1可能是CRKP诱导的肺炎发生这些变化的重要因素。因此，HMGB1可能为减少CRKP肺炎中的细菌感染和肺部炎症提供一个治疗靶点。
